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Cell No. : Cell Name
RCB4476 : Ba/F3  update : 2020/09/28
CommentMouse pro-B cell line derived from C3H strain. IL-3 dependent. Information
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Tani, Kenzaburo
Year of deposit 2012
Animal mouse < Mammals
Strain name C3H
Tissue bone marrow
Classification other
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 10ng/ml mouse IL-3
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
4829  Daley GQ1, Baltimore D.  Transformation of an interleukin 3-dependent hematopoietic cell line by the chronic myelogenous leukemia-specific P210bcr/abl protein.  Proc Natl Acad Sci U S A  1988  85(23):9312-6  PubMed ID: 3143116
4828  Palacios R, Steinmetz M.  Il-3-dependent mouse clones that express B-220 surface antigen, contain Ig genes in germ-line configuration, and generate B lymphocytes in vivo.  Cell  1985  41(3):727-34  PubMed ID: 3924409
4827  Palacios R, Henson G, Steinmetz M, McKearn JP.  Interleukin-3 supports growth of mouse pre-B-cell clones in vitro.  Nature  1984  309(5964):126-31  PubMed ID: 6201749

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