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Cell No. : Cell Name
RCB4476 : Ba/F3  update : 2021/05/20
CommentMouse pro-B cell line derived from C3H strain. IL-3 dependent. Information
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Tani, Kenzaburo
Year of deposit 2012
Animal _mouse < Mammals
Strain name C3H
Tissue bone marrow
Classification other
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0161
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 10ng/ml mouse IL-3
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 1


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Reference
4829  Daley GQ1, Baltimore D.  Transformation of an interleukin 3-dependent hematopoietic cell line by the chronic myelogenous leukemia-specific P210bcr/abl protein.  Proc Natl Acad Sci U S A  1988  85(23):9312-6  PubMed ID: 3143116
4828  Palacios R, Steinmetz M.  Il-3-dependent mouse clones that express B-220 surface antigen, contain Ig genes in germ-line configuration, and generate B lymphocytes in vivo.  Cell  1985  41(3):727-34  PubMed ID: 3924409
4827  Palacios R, Henson G, Steinmetz M, McKearn JP.  Interleukin-3 supports growth of mouse pre-B-cell clones in vitro.  Nature  1984  309(5964):126-31  PubMed ID: 6201749

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User's Publication
13269  Kang JH, Katsikis G, Li Z, Sapp KM, Stockslager MA, Lim D, Vander Heiden MG, Yaffe MB, Manalis SR, Miettinen TP.  Monitoring and modeling of lymphocytic leukemia cell bioenergetics reveals decreased ATP synthesis during cell division.  Nat Commun  2020    PubMed ID: 33020492



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