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Cell No. : Cell Name
RCB4475 : SMP30/GNL-KO  update : 2024/07/09
Comment
Comment from the depositorDeletion of the ascorbic acid synthesis enzyme gluconolactonase gene, and introduction of the temperature-sensitive SV40T antigen tsA58 gene.
Terms and conditions1) The BIOLOGICAL RESOURCE is provided as a service to research community. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biochem 2011 150(6):671-8) designated by the DEPOSITOR is required.
Remarks
approver's address
×
English
Address
Tokyo Metropolitan Institute for Geriatrics and Gerontology
35-2 Sakaecyo, Itabashiku, Tokyo, 173-0015
Dr.Ishigami, Akihito
Fax. +81-3-3579-4776
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ISHIGAMI, Akihito
Originator ISHIGAMI, Akihito
Year of deposit 2012
Animal _mouse < Mammals
Strain name SMP30/GNL KO
Genus Mus
Species musculus
Age at sampling 6 months
Tissue liver (parenchymal cells)
Genetics アスコルピン酸合成酵素グルコノラク上ナーゼ遺伝子欠損
Classification transformed
Recombinant recombinant
Exogene SV40 large T antigen tsA58 gene
Recombinant virus No
Lifespan infinite
Morphology epithelial-like
インターフェロンγによる増殖促進
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium William's medium E (Invitrogen), 5%FCS, 1ng/ml recombinant mouse interferon-γ (Thermo Fisher Scientific), 10ng/ml epidermal growth factor (Biomedical Technologies, Inc.), 10ng/ml insulin, 5.5ng/ml transferring, 0.0067ng/ml sodium selenite, 10mM nicotinamide, 0.4mg/l dexamethasone, 2mM L-glutamine William's medium E + 5% FBS + 1ng/ml mouse IFN-γ + 10ng/ml EGF + 10ng/ml Insulin + 5.5ng/ml Transferring + 0.0067ng/ml Sodium selenite + 10mM Nicotinamide + 0.4mg/l Dexamethasone + 2mM L-Glutamine
Antibiotics 100U/ml penicillin and 100mg/ml streptomycin Free
Passage method 0.25% trypsin 0.25% Trypsin
Coating dish bovine type l collagen (Sigma-Aldrich) bovine type l collagen
Culture information Passage cell No 2.5×10 5 cells/ml
Passage ratio 1 : 4-8 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃ 33 ℃
CO2 concentration 5 % 5 %
Freeze medium Bambanker CELLBANKER 1
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 2
User's Publication 0


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Reference
16314  Akiko Amano, Setsuko Handa, Toshiro Aigaki, Kazuhiro Shigemoto, Naoki Maruyama, Akihito Ishigami  Establishment and characterization of hepatocytes from an Immortomouse/SMP30/GNL knockout mouse hybrid lacking vitamin C to study vitamin C transport  J Biochem  2011  150(6):671-8  PubMed ID: 21903636   DOI: 10.1093/jb/mvr109
16315  Akihito Ishigami 1, Toshiko Fujita, Setsuko Handa, Takuji Shirasawa, Haruhiko Koseki, Tsuneo Kitamura, Nobuyuki Enomoto, Nobuhiro Sato, Tatsuo Shimosawa, Naoki Maruyama  Senescence marker protein-30 knockout mouse liver is highly susceptible to tumor necrosis factor-alpha- and Fas-mediated apoptosis  Am J Pathol  2002  161(4):1273-81  PubMed ID: 12368201   DOI: 10.1016/s0002-9440(10)64404-5

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