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Cell No. : Cell Name
RCB4475 : SMP30/GNL-KO update : 2024/11/11 |
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| Comment | ||||
| Comment from the depositor | Deletion of the ascorbic acid synthesis enzyme gluconolactonase gene, and introduction of the temperature-sensitive SV40T antigen tsA58 gene. | |||
| Terms and conditions | 1) The BIOLOGICAL RESOURCE is provided as a service to research community. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biochem 2011 150(6):671-8) designated by the DEPOSITOR is required. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | ISHIGAMI, Akihito | ||
| Originator | ISHIGAMI, Akihito | |||
| Year of deposit | 2012 | |||
| Animal | _mouse < Mammals | |||
| Strain name | SMP30/GNL KO | |||
| Genus | Mus | |||
| Species | musculus | |||
| Age at sampling | 6 months | |||
| Tissue | liver (parenchymal cells) | |||
| Genetics | アスコルピン酸合成酵素グルコノラク上ナーゼ遺伝子欠損 | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | SV40 large T antigen tsA58 gene | |||
| Recombinant virus | No | Lifespan | infinite | |
| Morphology | epithelial-like | |||
| インターフェロンγによる増殖促進 | ||||
| Medium | Medium List | Culture type | Adherent cells | Adherent cells |
| Culture medium | William's medium E (Invitrogen), 5%FCS, 1ng/ml recombinant mouse interferon-γ (Thermo Fisher Scientific), 10ng/ml epidermal growth factor (Biomedical Technologies, Inc.), 10ng/ml insulin, 5.5ng/ml transferring, 0.0067ng/ml sodium selenite, 10mM nicotinamide, 0.4mg/l dexamethasone, 2mM L-glutamine | William's medium E + 5% FBS + 1ng/ml mouse IFN-γ + 10ng/ml EGF + 10ng/ml Insulin + 5.5ng/ml Transferring + 0.0067ng/ml Sodium selenite + 10mM Nicotinamide + 0.4mg/l Dexamethasone + 2mM L-Glutamine | ||
| Antibiotics | 100U/ml penicillin and 100mg/ml streptomycin | Free | Passage method | 0.25% trypsin | 0.25% Trypsin |
| Coating dish | bovine type l collagen (Sigma-Aldrich) | bovine type l collagen | Culture information | Passage cell No | 2.5×10 5 cells/ml |
| Passage ratio | 1 : 4-8 split | |||
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 33 ℃ | 33 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | Bambanker | CELLBANKER 1 | ||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| SSLP(mouse) | OK | |||
| Reference information | Reference | 2 | ||
| User's Publication | 0 | |||
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