Back Back
Cell No. : Cell Name
RCB4474 : Ba/F3-CL1  update : 2024/01/17
CommentMouse pro-B cell line derived from C3H strain. IL-3 dependent. After cloning (RCB0805 Ba/F3). Information
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor RIKEN BRC
Year of deposit 2012
Original cell RCB0805
Cloning (depositor) Yes
Animal _mouse < Mammals
Strain name C3H
Tissue bone marrow
Classification other
Memo_1 RCB0805 : Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_T765
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS + 10ng/ml mouse IL-3
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
deposit info
lot info
Reference information Reference 3
User's Publication 8

To topTop
4829  Daley GQ1, Baltimore D.  Transformation of an interleukin 3-dependent hematopoietic cell line by the chronic myelogenous leukemia-specific P210bcr/abl protein.  Proc Natl Acad Sci U S A  1988  85(23):9312-6  PubMed ID: 3143116   DOI: 10.1073/pnas.85.23.9312
4828  Palacios R, Steinmetz M.  Il-3-dependent mouse clones that express B-220 surface antigen, contain Ig genes in germ-line configuration, and generate B lymphocytes in vivo.  Cell  1985  41(3):727-34  PubMed ID: 3924409   DOI: 10.1016/s0092-8674(85)80053-2
4827  Palacios R, Henson G, Steinmetz M, McKearn JP.  Interleukin-3 supports growth of mouse pre-B-cell clones in vitro.  Nature  1984  309(5964):126-31  PubMed ID: 6201749   DOI: 10.1038/309126a0

To topTop
User's Publication
19766  Hamada A, Suda K, Nishino M, Obata K, Oiki H, Fukami T, Fukuda S, Fujino T, Ohara S, Koga T, Chiba M, Shimoji M, Ito M, Takemoto T, Soh J, Tsutani Y, Mitsudomi T.  Secondary Mutations of the EGFR Gene That Confer Resistance to Mobocertinib in EGFR Exon 20 Insertion  J Thorac Oncol  2024  19(1):71-79  PubMed ID: 37666482   DOI: 10.1016/j.jtho.2023.08.029
19833  Arai A, Yoshimitsu M, Otsuka M, Ito Y, Miyazono T, Nakano N, Obama K, Nakashima H, Hanada S, Owatari S, Nakamura D, Tokunaga M, Kamada Y, Utsunomiya A, Haraguchi K, Hayashida M, Fujino S, Odawara J, Tabuchi T, Suzuki S, Hamada H, Kawamoto Y, Uchida Y, Hachiman M, Ishitsuka K.  Identification of putative noncanonical driver mutations in patients with essential thrombocythemia  Eur J Haematol  2023  110(6):639-647  PubMed ID: 36811253   DOI: 10.1111/ejh.13945
20412  Fujino T, Suda K, Sakai K, Murakami I, Shimizu S, Ohara S, Koga T, Hamada A, Soh J, Nishio K, Mitsudomi T.  Intra-tumor and Inter-tumor Heterogeneity in MET Exon 14 Skipping Mutations and Co-mutations in Pulmonary Pleomorphic Carcinomas.  Clin Lung Cancer  2022    PubMed ID: 34702670   DOI: 10.1016/j.cllc.2021.09.005
20407  Hamada A, Suda K, Koga T, Fujino T, Nishino M, Ohara S, Chiba M, Shimoji M, Takemoto T, Soh J, Uchida T, Mitsudomi T.  In vitro validation study of HER2 and HER4 mutations identified in an ad hoc secondary analysis of the LUX-Lung 8 randomized clinical trial.  Lung Cancer  2021  162:79-85  PubMed ID: 34741886   DOI: 10.1016/j.lungcan.2021.10.014
20411  Ota Y, Yoda H, Inoue T, Watanabe T, Shinozaki Y, Takatori A, Nagase H.  Targeting anaplastic lymphoma kinase (ALK) gene alterations in neuroblastoma by using alkylating pyrrole-imidazole polyamides.  PLoS One  2021  16(9):e0257718  PubMed ID: 34591871   DOI: 10.1371/journal.pone.0257718
17935  Saito Y, Koya J, Araki M, Kogure Y, Shingaki S, Tabata M, McClure MB, Yoshifuji K, Matsumoto S, Isaka Y, Tanaka H, Kanai T, Miyano S, Shiraishi Y, Okuno Y, Kataoka K.  Landscape and function of multiple mutations within individual oncogenes  Nature  2020  582(7810):95-99  PubMed ID: 32494066   DOI: 10.1038/s41586-020-2175-2
11758  Yoshimitsu M, Hachiman M, Uchida Y, Arima N, Arai A, Kamada Y, Shide K, Ito M, Shimoda K, Ishitsuka K.  Essential thrombocytosis attributed to JAK2-T875N germline mutation.  Int. J. Hematol.  2019  10(5):584-590  PubMed ID: 31428969   DOI: 10.1007/s12185-019-02725-8
12392  Kashiwagi S, Fujioka Y, Kondo T, Satoh AO, Yoshida A, Fujioka M, Sasajima H, Amano M, Teshima T, Ohba Y.  Localization of BCR-ABL to Stress Granules Contributes to Its Oncogenic Function  Cell Struct Funct  2019  44(2):195-204  PubMed ID: 31735741   DOI: 10.1247/csf.19033

Back Back Return Top Page