Cell No. : Cell Name
RCB4473 : F6B3
update : 2023/10/16
|
| Comment | Mouse brain tumors induced with Rous sarcom. |
| Comment from the depositor | |
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biomedical Research 1984 5:517-520 ) designated by the DEPOSITOR is required. 2) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURSE.
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| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
KUMANISHI, Toshiro
|
| Originator |
SHIMBO, Yoshikatsu
|
| Year of deposit |
2012
|
| Another name |
SE-C3H/St glioma
|
| Cloning (depositor) |
Yes
|
| _Date (depositor) |
1980
|
| _Method (depositor) |
limitting dilution
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H/St
|
| Genus |
Mus
|
| Species |
Musculus
|
| Gender |
Unknown
|
| Tissue |
subcutaneously passaged mouse brain tumors
|
| Primary focus |
brain
|
| Disease name |
Brain tumor
|
| Metastatic ability |
No
|
|
SR-RSV-induced brain tumor cells
|
| Classification |
transformed
|
|
Lifespan |
infinite
|
| Morphology |
other
(biolar)
|
| Differentiation |
GFAP (Glial fibrillary acidic protein)
|
| Cellosaurus(Expasy) |
CVCL_4Y23
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
|
Culture information
|
Passage ratio |
|
1 : 6 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Reference information |
Reference |
2
|
| User's Publication |
0
|
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