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Cell No. : Cell Name
RCB4473 : F6B3  update : 2022/07/13
CommentMouse brain tumors induced with Rous sarcom.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biomedical Research 1984 5:517-520 ) designated by the DEPOSITOR is required. 2) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURSE.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KUMANISHI, Toshiro
Originator SHIMBO, Yoshikatsu
Year of deposit 2012
Another name SE-C3H/St glioma
Cloning (depositor) Yes
_Date (depositor) 1980
_Method (depositor) limitting dilution
Animal mouse < Mammals
Strain name C3H/St
Genus Mus
Species Musculus
Gender Unknown
Tissue subcutaneously passaged mouse brain tumors
Primary focus brain
Disease name Brain tumor
Metastastatic ability No
SR-RSV-induced brain tumor cells
Classification transformed
Lifespan infinite
Morphology other (biolar)
Differentiation GFAP (Glial fibrillary acidic protein)
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 2
User's Publication 0


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Reference
921  YOSHIKATSU SHIMBO, TOSHIRO KUMANISHI, KAZUNORI YAMAZAKI, FUSAHIRO IKUTA, KENTARO SEKIGUCHI  Isolation of glial fibrillary acidic protein-positive clonal mouse glioma cell line  Biomedical Research  1984  5:517-520    DOI: 10.2220/biomedres.5.517
923  T Kumanishi  Brain tumors induced with Rous sarcoma virus, Schmidt-Ruppin strain. I. Induction of brain tumors in adult mice with Rous chicken sarcoma cells  Jpn J Exp Med  1967  37(5):461-74.  PubMed ID: 4301953  

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