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Cell No. : Cell Name
RCB4472 : IN-IC  update : 2022/05/30
CommentRat cell line derived from kidney.
Comment from the depositor
Terms and conditions1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Am Soc Nephrol 2011;22:673-80) designated by the DEPOSITOR is required. 3) It is prohibited to transfer the cell to another person without getting permission of the original cell holder.
approver's address
Kitasato University
Kitasato Institute Medical Center Hospital
6-100 Arai, Kitamoto-shi, Saitama, 364-8501 Japan
Fax. +81-48-593-1239
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Nonoguchi, Hiroshi
Originator Izumi, Yuichiro & Nonoguchi, Hiroshi
Year of deposit 2012
Animal rat < Mammals
Genus Rattus
Species norvegicus
Gender Male
Age at sampling 8-12 weeks
Tissue kidney
Classification transformed
Recombinant recombinant
Exogene tsA58 Tg
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_VQ62
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS + 1μg/ml human Insulin + 10μg/ml bovine apo-Transferrin + 0.5μg/ml Hydrocortisone + 6.5ng/ml Triiodo-Thyronine + 10ng/ml human EGF
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Mycoplasma (-)
Animal PCR OK
deposit info
lot info
Reference information Reference 4
User's Publication 0

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5505  Izumi Y, Inoue H, Nakayama Y, Eguchi K, Yasuoka Y, Matsuo N, Nonoguchi H, Kakizoe Y, Kuwabara T, Mukoyama M.  TSS-Seq analysis of low pH-induced gene expression in intercalated cells in the renal collecting duct.  PLoS One  2017  12(8):e0184185  PubMed ID: 28859164   DOI: 10.1371/journal.pone.0184185
5504  Nagai T, Yasuoka Y, Izumi Y, Horikawa K, Kimura M, Nakayama Y, Uematsu T, Fukuyama T, Yamazaki T, Kohda Y, Hasuike Y, Nanami M, Kuragano T, Kobayashi N, Obinata M, Tomita K, Tanoue A, Nakanishi T, Kawahara K, Nonoguchi H.  Reevaluation of erythropoietin production by the nephron.  Biochem Biophys Res Commun  2014  449(2):222-8  PubMed ID: 24832733   DOI: 10.1016/j.bbrc.2014.05.014
5503  Hori K, Nagai T, Izumi Y, Kimura M, Hasuike Y, Nakayama Y, Nanami M, Tokuyama M, Otaki Y, Kuragano T, Kohda Y, Obinata M, Kawahara K, Tanoue A, Tomita K, Nakanishi T, Nonoguchi H.  Vasopressin V1a receptor is required for nucleocytoplasmic transport of mineralocorticoid receptor.  Am J Physiol Renal Physiol  2012  303(7):F1080-8  PubMed ID: 22811487   DOI: 10.1152/ajprenal.00052.2012
5502  Izumi Y, Hori K, Nakayama Y, Kimura M, Hasuike Y, Nanami M, Kohda Y, Otaki Y, Kuragano T, Obinata M, Kawahara K, Tanoue A, Tomita K, Nakanishi T, Nonoguchi H.  Aldosterone requires vasopressin V1a receptors on intercalated cells to mediate acid-base homeostasis.  J Am Soc Nephrol  2011  22(4):673-80  PubMed ID: 21415155   DOI: 10.1681/ASN.2010050468

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