Cell No. : Cell Name RCB4469 : TIG-7  update : 2025/01/16
Comment			Human lung-derived adherent cells. The origin is not cancer. 25-35PDL.
Comment from the depositor
Terms and conditions			1) Basically, there is no restriction regarding academic use. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	KAJI, Kazuhiko
		Originator	KAJI, Kazuhiko
		Year of deposit	2011
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Male
		Age at sampling	11 weeks embryo
		Tissue	lung
		Classification	normal
		Lifespan	finite
		Crisis PDL	66-80 PDL
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_E940
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		MEM + 30% FBS + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		STR(human)		OK
Reference information		Reference	6
		User's Publication	1

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Reference

18629  Yamamoto H, Iguchi K, Unno K, Kaji K, Hoshino M.  Expression and release of progalanin in fibroblasts  Regul Pept  2014  194-195:55-62.  PubMed ID: 25229126   DOI: 10.1016/j.regpep.2014.09.004

8980  M Miyazaki, E Gohda, K Kaji, M Namba  Increased Hepatocyte Growth Factor Production by Aging Human Fibroblasts Mainly Due to Autocrine Stimulation by interleukin-1  Biochem Biophys Res Commun  1998  246 (1), 255-60  PubMed ID: 9600102   DOI: 10.1006/bbrc.1998.8593

8985  S Masuda, S Araki, T Yamamoto, K Kaji, H Hayashi  Purification of a Vascular Apoptosis-Inducing Factor From Hemorrhagic Snake Venom  Biochem Biophys Res Commun  1997  235 (1), 59-63  PubMed ID: 9196035   DOI: 10.1006/bbrc.1997.6728

8971  K Murakami-Murofushi , K Kaji, K Kano, M Fukuda, M Shioda, H Murofushi  Inhibition of Cell Proliferation by a Unique Lysophosphatidic Acid, PHYLPA, Isolated From Physarum Polycephalum: Signaling Events of Antiproliferative Action by PHYLPA  Cell Struct Funct  1993  18 (5), 363-70  PubMed ID: 8168160   DOI: 10.1247/csf.18.363

8984  Reiko Hirai, Kazuhiko Kaji  SUSCEPTIBILITY OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS TO TRANSFORMING GROWTH FACTOR β(TGFβ) AND THE EXPRESSION OF TGFβ RECEPTOR/BINDING PROTEINS ON THESE CELLS  TISSUE CULTURE RESEARCH COMMUNICATIONS,   1992  11:317-327    DOI: 10.11418/jtca1981.11.4_317

7430  Yamamoto K, Kaji K, Kondo H, Matsuo M, Shibata Y, Tasaki Y, Utakoji T, Ooka H.  A new human male diploid cell strain, TIG-7: its age-related changes and comparison with a matched female TIG-1 cell strain.  Exp Gerontol  1991  26(6):525-40  PubMed ID: 1800129   DOI: 10.1016/0531-5565(91)90071-s

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User's Publication

22524  Kasai F, Fukushima M, Miyagi Y, Nakamura Y.  Genetic diversity among the present Japanese population: evidence from genotyping of human cell lines established in Japan  Hum Cell  2024  37(4):944-950  PubMed ID: 38639832   DOI: 10.1007/s13577-024-01055-0