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Cell No. : Cell Name
RCB4469 : TIG-7  update : 2022/01/19
CommentHuman lung-derived adherent cells. The origin is not cancer.
Comment from the depositor
Terms and conditions1) Basically, there is no restriction regarding academic use. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KAJI, Kazuhiko
Originator KAJI, Kazuhiko
Year of deposit 2011
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 11 weeks embryo
Tissue lung
Classification normal
Lifespan finite
Crisis PDL 66-80 PDL
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_E940
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium MEM + 30% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Reference information Reference 5
User's Publication 0


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Reference
8980  M Miyazaki, E Gohda, K Kaji, M Namba  Increased Hepatocyte Growth Factor Production by Aging Human Fibroblasts Mainly Due to Autocrine Stimulation by interleukin-1  Biochem Biophys Res Commun  1998  246 (1), 255-60  PubMed ID: 9600102   DOI: 10.1006/bbrc.1998.8593
8985  S Masuda, S Araki, T Yamamoto, K Kaji, H Hayashi  Purification of a Vascular Apoptosis-Inducing Factor From Hemorrhagic Snake Venom  Biochem Biophys Res Commun  1997  235 (1), 59-63  PubMed ID: 9196035   DOI: 10.1006/bbrc.1997.6728
8971  K Murakami-Murofushi , K Kaji, K Kano, M Fukuda, M Shioda, H Murofushi  Inhibition of Cell Proliferation by a Unique Lysophosphatidic Acid, PHYLPA, Isolated From Physarum Polycephalum: Signaling Events of Antiproliferative Action by PHYLPA  Cell Struct Funct  1993  18 (5), 363-70  PubMed ID: 8168160   DOI: 10.1247/csf.18.363
8984  Reiko Hirai, Kazuhiko Kaji  SUSCEPTIBILITY OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS TO TRANSFORMING GROWTH FACTOR β(TGFβ) AND THE EXPRESSION OF TGFβ RECEPTOR/BINDING PROTEINS ON THESE CELLS  TISSUE CULTURE RESEARCH COMMUNICATIONS,   1992  11:317-327    DOI: 10.11418/jtca1981.11.4_317
7430  Yamamoto K, Kaji K, Kondo H, Matsuo M, Shibata Y, Tasaki Y, Utakoji T, Ooka H.  A new human male diploid cell strain, TIG-7: its age-related changes and comparison with a matched female TIG-1 cell strain.  Exp Gerontol  1991  26(6):525-40  PubMed ID: 1800129   DOI: 10.1016/0531-5565(91)90071-s

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