細胞番号 : 細胞名
RCB4468 : TIG-3
update : 2023/10/16
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| 細胞特性(Comment:英) | Human lung-derived adherent cells. The origin is not cancer. 25-35PDL. |
| 細胞特性(日) | ヒト肺由来付着性細胞。癌組織由来ではない。25-35 PDL。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | 細胞老化研究。ヒト正常細胞の標準細胞。 |
| 使用条件(英) | 1) Basically, there is no restriction regarding academic use. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| 使用条件(日) | 1) 学術研究における利用につきましては原則として使用制限はありません。2) 研究成果の公表にあたって謝辞の表明を必要とする。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
KAJI, Kazuhiko
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| 樹立者 |
kAJI, Kazuhiko
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| 寄託日 |
2011
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 人種 |
Japanese
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| 性別 |
Male
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| 齢 |
10 weeks embryo
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| 採取組織 |
lung
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| 細胞分類 |
normal
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|
細胞寿命 |
finite
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| 分裂寿命(PDL) |
84
PDL
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| 細胞形態 |
fibroblast-like
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| Cellosaurus(Expasy) |
CVCL_E939
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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MEM + 10% FBS
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin
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|
継代密度 |
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1 : 4 split
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| 継代・培地交換頻度 |
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Subculture : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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MEM + 30% FBS + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| 動物種PCR |
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OK
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| 個体識別検査 |
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OK
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| 文献情報 |
Reference(英) |
11件
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| Reference(日) |
1件
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| 利用者成果(英) |
6件
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| 利用者成果(日) |
0件
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| Reference(英) |
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18629
Yamamoto H, Iguchi K, Unno K, Kaji K, Hoshino M.
Expression and release of progalanin in fibroblasts
Regul Pept
2014
194-195:55-62.
PubMed ID: 25229126
DOI: 10.1016/j.regpep.2014.09.004
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8983
Mariko Nakano, Yasutaka Kakiuchi, Yukiko Shimada, Mika Ohyama, Yasuko Ogiwara, Narie Sasaki-Higashiyama, Naoko Yano, Fumi Ikeda, Ei Yamada, Akihiro Iwamatsu, Kazuo Kobayashi, Kiyoto Nishiyama, Sohsuke Ichikawa, Kazuhiko Kaji, Toshinori Ide, Hiromu Murofushi, Kimiko Murakami-Murofushi
MOV10 as a Novel Telomerase-Associated Protein
Biochem Biophys Res Commun
2009
388 (2), 328-32
PubMed ID: 19665004
DOI: 10.1016/j.bbrc.2009.08.002
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8973
T Toda, K Kaji, N Kimura
TMIG-2DPAGE: A New Concept of Two-Dimensional Gel Protein Database for Research on Aging
Electrophoresis
1998
19 (2), 344-8
PubMed ID: 9548301
DOI: 10.1002/elps.1150190232
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8974
K Ohneda, M Fukuda, N Shimada, N Ishikawa, T Ichou, K Kaji, T Toyota, N Kimura
Increased Expression of Nucleoside Diphosphate kinases/nm23 in Human Diploid Fibroblasts Transformed by SV40 Large T Antigen or 60Co Irradiation
FEBS Lett
1994
348 (3), 273-7
PubMed ID: 8034053
DOI: 10.1016/0014-5793(94)00623-7
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8971
K Murakami-Murofushi , K Kaji, K Kano, M Fukuda, M Shioda, H Murofushi
Inhibition of Cell Proliferation by a Unique Lysophosphatidic Acid, PHYLPA, Isolated From Physarum Polycephalum: Signaling Events of Antiproliferative Action by PHYLPA
Cell Struct Funct
1993
18 (5), 363-70
PubMed ID: 8168160
DOI: 10.1247/csf.18.363
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18631
Kondo H, Yonezawa Y.
Changes in the migratory ability of human lung and skin fibroblasts during in vitro aging and in vivo cellular senescence
Mech Ageing Dev
1992
63(3):223-33.
PubMed ID: 1614223
DOI: 10.1016/0047-6374(92)90001-t
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8982
Y Shimada, H Ito, K Kaji, M Fukuda
Tumor Necrosis Factor Reduces Lifespan of Human Endothelial Cells in Vitro
Mech Ageing Dev
1990
55 (3), 245-54
PubMed ID: 2232916
DOI: 10.1016/0047-6374(90)90152-6
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8977
Y Yokoyama, K Kano, K Kaji, Y Seyama
Purification and Characterization of a Growth Factor From Guinea Pig Harderian Gland
J Biol Chem
1989
, 264 (29), 17058-63
PubMed ID: 2551893
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8978
S Kotani, H Murofushi, S Maekawa, H Aizawa, K Kaji, H Sakai
Identification of the 190 kD Microtubule-Associated Protein in Cultured Fibroblasts and Its Association With Interphase and Mitotic Microtubules
Cell Struct Funct
1987
12 (1), 1-9
PubMed ID: 3552259
DOI: 10.1247/csf.12.1
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8975
K Kaji, M Matsuo
Responsiveness of Human Lung Diploid Fibroblast Ageing in Vitro to Epidermal Growth Factor: Saturation Density and Lifespan
Mech Ageing Dev
1983
22 (2), 129-33
PubMed ID: 6605462
DOI: 10.1016/0047-6374(83)90106-9
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7427
Matsuo M, Kaji K, Utakoji T, Hosoda K.
Ploidy of human embryonic fibroblasts during in vitro aging.
J Gerontol
1982
37(1):33-7
PubMed ID: 7053395
DOI: 10.1093/geronj/37.1.33
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| 利用者成果(英) |
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21615
Shimura T, Sunaga K, Yamazaki M, Honoka N, Sasatani M, Kamiya K, Ushiyama A.
Nuclear DNA damage-triggered ATM-dependent AMPK activation regulates the mitochondrial radiation response.
Int J Radiat Biol
2024
1-11
PubMed ID: 38166485
DOI: 10.1080/09553002.2023.2295297
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21373
Suzuki MM, Iijima K, Ogami K, Shinjo K, Murofushi Y, Xie J, Wang X, Kitano Y, Mamiya A, Kibe Y, Nishimura T, Ohka F, Saito R, Sato S, Kobayashi J, Yao R, Miyata K, Kataoka K, Suzuki HI, Kondo Y.
TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation.
Nat Commun
2023
14(1):4521
PubMed ID: 37607907
DOI: 10.1038/s41467-023-40243-8
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20512
Shimura T, Nakashiro C, Fujiwara K, Shiga R, Sasatani M, Kamiya K, Ushiyama A.
Radiation affects glutathione redox reaction by reduced glutathione peroxidase activity in human fibroblasts.
J Radiat Res
2022
PubMed ID: 34977941
DOI: 10.1093/jrr/rrab122
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20843
Shimura T, Zaharieva E, Sasatani M, Kawai H, Kamiya K, Ushiyama A.
ACTIVATION OF HUMAN FIBROBLASTS BY CHRONIC RADIATION RATHER THAN ACUTE RADIATION.
Radiat Prot Dosimetry
2022
198(13-15):1098-1103
PubMed ID: 36083750
DOI: 10.1093/rpd/ncac065
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20884
Shindo A, Kusano M, Sakagami H, Amano S, Inomata M, Abe M, Okazawa M, Ooka T.
Comparison of UVC Sensitivity and Dectin-2 Expression Between Malignant and Non-malignant Cells.
In Vivo
2022
36(5):2116-2125
PubMed ID: 36099100
DOI: 10.21873/invivo.12937
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20934
Shimura T, Totani R, Ogasawara H, Inomata K, Sasatani M, Kamiya K, Ushiyama A.
Effects of oxygen on the response of mitochondria to X-irradiation and reactive oxygen species-mediated fibroblast activation.
Int J Radiat Biol
2022
1-10
PubMed ID: 36383181
DOI: 10.1080/09553002.2023.2142980
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