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Cell No. : Cell Name
RCB4468 : TIG-3  update : 2023/05/26
CommentHuman lung-derived adherent cells. The origin is not cancer. 25-35PDL.
Comment from the depositor
Terms and conditions1) Basically, there is no restriction regarding academic use. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor KAJI, Kazuhiko
Originator kAJI, Kazuhiko
Year of deposit 2011
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 10 weeks embryo
Tissue lung
Classification normal
Lifespan finite
Crisis PDL 84 PDL
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_E939
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium MEM + 30% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Reference information Reference 9
User's Publication 5

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8983  Mariko Nakano, Yasutaka Kakiuchi, Yukiko Shimada, Mika Ohyama, Yasuko Ogiwara, Narie Sasaki-Higashiyama, Naoko Yano, Fumi Ikeda, Ei Yamada, Akihiro Iwamatsu, Kazuo Kobayashi, Kiyoto Nishiyama, Sohsuke Ichikawa, Kazuhiko Kaji, Toshinori Ide, Hiromu Murofushi, Kimiko Murakami-Murofushi  MOV10 as a Novel Telomerase-Associated Protein  Biochem Biophys Res Commun  2009  388 (2), 328-32  PubMed ID: 19665004   DOI: 10.1016/j.bbrc.2009.08.002
8973  T Toda, K Kaji, N Kimura  TMIG-2DPAGE: A New Concept of Two-Dimensional Gel Protein Database for Research on Aging  Electrophoresis   1998  19 (2), 344-8   PubMed ID: 9548301   DOI: 10.1002/elps.1150190232
8974  K Ohneda, M Fukuda, N Shimada, N Ishikawa, T Ichou, K Kaji, T Toyota, N Kimura  Increased Expression of Nucleoside Diphosphate kinases/nm23 in Human Diploid Fibroblasts Transformed by SV40 Large T Antigen or 60Co Irradiation  FEBS Lett  1994  348 (3), 273-7  PubMed ID: 8034053   DOI: 10.1016/0014-5793(94)00623-7
8971  K Murakami-Murofushi , K Kaji, K Kano, M Fukuda, M Shioda, H Murofushi  Inhibition of Cell Proliferation by a Unique Lysophosphatidic Acid, PHYLPA, Isolated From Physarum Polycephalum: Signaling Events of Antiproliferative Action by PHYLPA  Cell Struct Funct  1993  18 (5), 363-70  PubMed ID: 8168160   DOI: 10.1247/csf.18.363
8982  Y Shimada, H Ito, K Kaji, M Fukuda  Tumor Necrosis Factor Reduces Lifespan of Human Endothelial Cells in Vitro  Mech Ageing Dev  1990  55 (3), 245-54  PubMed ID: 2232916   DOI: 10.1016/0047-6374(90)90152-6
8977  Y Yokoyama, K Kano, K Kaji, Y Seyama  Purification and Characterization of a Growth Factor From Guinea Pig Harderian Gland  J Biol Chem  1989  , 264 (29), 17058-63  PubMed ID: 2551893  
8978  S Kotani, H Murofushi, S Maekawa, H Aizawa, K Kaji, H Sakai  Identification of the 190 kD Microtubule-Associated Protein in Cultured Fibroblasts and Its Association With Interphase and Mitotic Microtubules  Cell Struct Funct   1987  12 (1), 1-9  PubMed ID: 3552259   DOI: 10.1247/csf.12.1
8975  K Kaji, M Matsuo  Responsiveness of Human Lung Diploid Fibroblast Ageing in Vitro to Epidermal Growth Factor: Saturation Density and Lifespan  Mech Ageing Dev  1983  22 (2), 129-33  PubMed ID: 6605462   DOI: 10.1016/0047-6374(83)90106-9
7427  Matsuo M, Kaji K, Utakoji T, Hosoda K.  Ploidy of human embryonic fibroblasts during in vitro aging.  J Gerontol  1982  37(1):33-7  PubMed ID: 7053395   DOI: 10.1093/geronj/37.1.33

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User's Publication
21373  Suzuki MM, Iijima K, Ogami K, Shinjo K, Murofushi Y, Xie J, Wang X, Kitano Y, Mamiya A, Kibe Y, Nishimura T, Ohka F, Saito R, Sato S, Kobayashi J, Yao R, Miyata K, Kataoka K, Suzuki HI, Kondo Y.  TUG1-mediated R-loop resolution at microsatellite loci as a prerequisite for cancer cell proliferation.  Nat Commun  2023  14(1):4521  PubMed ID: 37607907   DOI: 10.1038/s41467-023-40243-8
20512  Shimura T, Nakashiro C, Fujiwara K, Shiga R, Sasatani M, Kamiya K, Ushiyama A.  Radiation affects glutathione redox reaction by reduced glutathione peroxidase activity in human fibroblasts.  J Radiat Res  2022    PubMed ID: 34977941   DOI: 10.1093/jrr/rrab122
20843  Shimura T, Zaharieva E, Sasatani M, Kawai H, Kamiya K, Ushiyama A.  ACTIVATION OF HUMAN FIBROBLASTS BY CHRONIC RADIATION RATHER THAN ACUTE RADIATION.  Radiat Prot Dosimetry  2022  198(13-15):1098-1103  PubMed ID: 36083750   DOI: 10.1093/rpd/ncac065
20884  Shindo A, Kusano M, Sakagami H, Amano S, Inomata M, Abe M, Okazawa M, Ooka T.  Comparison of UVC Sensitivity and Dectin-2 Expression Between Malignant and Non-malignant Cells.  In Vivo  2022  36(5):2116-2125  PubMed ID: 36099100   DOI: 10.21873/invivo.12937
20934  Shimura T, Totani R, Ogasawara H, Inomata K, Sasatani M, Kamiya K, Ushiyama A.  Effects of oxygen on the response of mitochondria to X-irradiation and reactive oxygen species-mediated fibroblast activation.  Int J Radiat Biol  2022  1-10  PubMed ID: 36383181   DOI: 10.1080/09553002.2023.2142980

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