Cell No. : Cell Name
RCB4467 : TIG-1
update : 2024/05/17
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Comment | Human lung-derived adherent cells. The origin is not cancer. 25-35PDL. |
Comment from the depositor | |
Terms and conditions | 1) Basically, there is no restriction regarding academic use. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
KAJI, Kazuhiko
|
Originator |
KAJI, Kazuhiko
|
Year of deposit |
2011
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
|
Race |
Japanese
|
Gender |
Female
|
Age at sampling |
5 months embryo
|
Tissue |
lung
|
Classification |
normal
|
|
Lifespan |
finite
|
Crisis PDL |
62-72
PDL
|
Morphology |
fibroblast-like
|
Cellosaurus(Expasy) |
CVCL_0560
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
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Adherent cells
|
Culture medium |
|
MEM + 10% FBS
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Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 4 split
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SC frequency |
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Subculture : 2 times/week
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Temperature |
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37
℃
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CO2 concentration |
|
5
%
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Freeze medium |
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MEM + 30% FBS + 10% DMSO
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Freezing method |
|
Slow freezing
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Mycoplasma |
|
(-)
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Animal PCR |
|
OK
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STR(human) |
|
OK
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Reference information |
Reference |
7
|
User's Publication |
1
|
Reference |
18629
Yamamoto H, Iguchi K, Unno K, Kaji K, Hoshino M.
Expression and release of progalanin in fibroblasts
Regul Pept
2014
194-195:55-62.
PubMed ID: 25229126
DOI: 10.1016/j.regpep.2014.09.004
|
18631
Kondo H, Yonezawa Y.
Changes in the migratory ability of human lung and skin fibroblasts during in vitro aging and in vivo cellular senescence
Mech Ageing Dev
1992
63(3):223-33.
PubMed ID: 1614223
DOI: 10.1016/0047-6374(92)90001-t
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8981
Y Yonezawa, H Kondo, R Hirai, K Kaji, K Nishikawa
Species-specific Differences in the Mitogenic Activity of Heparin-Binding Growth Factors in the Sera of Various Mammals
Experientia
1992
48 (3), 239-45
PubMed ID: 1547852
DOI: 10.1007/bf01930464
|
8979
H Kondo, T A Nomaguchi, Y Sakurai, Y Yonezawa, K Kaji, M Matsuo, H Okabe
Effects of Serum From Human Subjects of Various Ages on Proliferation of Human Lung and Skin Fibroblasts
Exp Cell Res
1988
178 (2), 287-95
PubMed ID: 3169131
DOI: 10.1016/0014-4827(88)90399-0
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8975
K Kaji, M Matsuo
Responsiveness of Human Lung Diploid Fibroblast Ageing in Vitro to Epidermal Growth Factor: Saturation Density and Lifespan
Mech Ageing Dev
1983
22 (2), 129-33
PubMed ID: 6605462
DOI: 10.1016/0047-6374(83)90106-9
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7415
Ohashi M, Aizawa S, Ooka H, Ohsawa T, Kaji K, Kondo H, Kobayashi T, Noumura T, Matsuo M, Mitsui Y, Murota S, Yamamoto K, Ito H, Shimada H, Utakoji T.
A new human diploid cell strain, TIG-1, for the research on cellular aging.
Exp Gerontol
1980
15(2):121-33
PubMed ID: 7389832
DOI: 10.1016/0531-5565(80)90083-2
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8976
K Kaji, M Matsuo
A Low-Density Inoculation Method for the Serial Subcultivation of Human Diploid Fibroblasts: An Efficient Model System for the Study of Cellular Ageing
Mech Ageing Dev
1980
13 (3), 219-25
PubMed ID: 7421300
DOI: 10.1016/0047-6374(80)90034-2
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