Cell No. : Cell Name RCB4467 : TIG-1  update : 2024/05/17
Comment			Human lung-derived adherent cells. The origin is not cancer. 25-35PDL.
Comment from the depositor
Terms and conditions			1) Basically, there is no restriction regarding academic use. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	KAJI, Kazuhiko
		Originator	KAJI, Kazuhiko
		Year of deposit	2011
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	5 months embryo
		Tissue	lung
		Classification	normal
		Lifespan	finite
		Crisis PDL	62-72 PDL
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_0560
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		MEM + 30% FBS + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		STR(human)		OK
Reference information		Reference	7
		User's Publication	1

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Reference

18629  Yamamoto H, Iguchi K, Unno K, Kaji K, Hoshino M.  Expression and release of progalanin in fibroblasts  Regul Pept  2014  194-195:55-62.  PubMed ID: 25229126   DOI: 10.1016/j.regpep.2014.09.004

18631  Kondo H, Yonezawa Y.  Changes in the migratory ability of human lung and skin fibroblasts during in vitro aging and in vivo cellular senescence  Mech Ageing Dev  1992  63(3):223-33.  PubMed ID: 1614223   DOI: 10.1016/0047-6374(92)90001-t

8981  Y Yonezawa, H Kondo, R Hirai, K Kaji, K Nishikawa  Species-specific Differences in the Mitogenic Activity of Heparin-Binding Growth Factors in the Sera of Various Mammals  Experientia  1992  48 (3), 239-45  PubMed ID: 1547852   DOI: 10.1007/bf01930464

8979  H Kondo, T A Nomaguchi, Y Sakurai, Y Yonezawa, K Kaji, M Matsuo, H Okabe  Effects of Serum From Human Subjects of Various Ages on Proliferation of Human Lung and Skin Fibroblasts  Exp Cell Res  1988  178 (2), 287-95  PubMed ID: 3169131   DOI: 10.1016/0014-4827(88)90399-0

8975  K Kaji, M Matsuo  Responsiveness of Human Lung Diploid Fibroblast Ageing in Vitro to Epidermal Growth Factor: Saturation Density and Lifespan  Mech Ageing Dev  1983  22 (2), 129-33  PubMed ID: 6605462   DOI: 10.1016/0047-6374(83)90106-9

7415  Ohashi M, Aizawa S, Ooka H, Ohsawa T, Kaji K, Kondo H, Kobayashi T, Noumura T, Matsuo M, Mitsui Y, Murota S, Yamamoto K, Ito H, Shimada H, Utakoji T.  A new human diploid cell strain, TIG-1, for the research on cellular aging.  Exp Gerontol  1980  15(2):121-33  PubMed ID: 7389832   DOI: 10.1016/0531-5565(80)90083-2

8976  K Kaji, M Matsuo  A Low-Density Inoculation Method for the Serial Subcultivation of Human Diploid Fibroblasts: An Efficient Model System for the Study of Cellular Ageing  Mech Ageing Dev  1980  13 (3), 219-25  PubMed ID: 7421300   DOI: 10.1016/0047-6374(80)90034-2

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User's Publication

20390  Fujita M, Sasada M, Eguchi M, Iyoda T, Okuyama S, Osawa T, Tsuzuranuki K, Sakamoto M, Hagihara Y, Matsumura M, Osada S, Kodama H, Higami Y, Fukai F.  Induction of cellular senescence in fibroblasts through β1-integrin activation by tenascin-C-derived peptide and its protumor effect.  Am J Cancer Res  2021  11(9):4364-4379  PubMed ID: 34659892