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Cell No. : Cell Name
RCB4467 : TIG-1  update : 2022/01/19
CommentHuman lung-derived adherent cells. The origin is not cancer.
Comment from the depositor
Terms and conditions1) Basically, there is no restriction regarding academic use. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KAJI, Kazuhiko
Originator KAJI, Kazuhiko
Year of deposit 2011
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 5 months embryo
Tissue lung
Classification normal
Lifespan finite
Crisis PDL 62-72 PDL
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0560
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium MEM + 30% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Reference information Reference 5
User's Publication 1


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Reference
8981  Y Yonezawa, H Kondo, R Hirai, K Kaji, K Nishikawa  Species-specific Differences in the Mitogenic Activity of Heparin-Binding Growth Factors in the Sera of Various Mammals  Experientia  1992  48 (3), 239-45  PubMed ID: 1547852   DOI: 10.1007/bf01930464
8979  H Kondo, T A Nomaguchi, Y Sakurai, Y Yonezawa, K Kaji, M Matsuo, H Okabe  Effects of Serum From Human Subjects of Various Ages on Proliferation of Human Lung and Skin Fibroblasts  Exp Cell Res  1988  178 (2), 287-95  PubMed ID: 3169131   DOI: 10.1016/0014-4827(88)90399-0
8975  K Kaji, M Matsuo  Responsiveness of Human Lung Diploid Fibroblast Ageing in Vitro to Epidermal Growth Factor: Saturation Density and Lifespan  Mech Ageing Dev  1983  22 (2), 129-33  PubMed ID: 6605462   DOI: 10.1016/0047-6374(83)90106-9
7415  Ohashi M, Aizawa S, Ooka H, Ohsawa T, Kaji K, Kondo H, Kobayashi T, Noumura T, Matsuo M, Mitsui Y, Murota S, Yamamoto K, Ito H, Shimada H, Utakoji T.  A new human diploid cell strain, TIG-1, for the research on cellular aging.  Exp Gerontol  1980  15(2):121-33  PubMed ID: 7389832   DOI: 10.1016/0531-5565(80)90083-2
8976  K Kaji, M Matsuo  A Low-Density Inoculation Method for the Serial Subcultivation of Human Diploid Fibroblasts: An Efficient Model System for the Study of Cellular Ageing  Mech Ageing Dev  1980  13 (3), 219-25  PubMed ID: 7421300   DOI: 10.1016/0047-6374(80)90034-2

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User's Publication
20390  Fujita M, Sasada M, Eguchi M, Iyoda T, Okuyama S, Osawa T, Tsuzuranuki K, Sakamoto M, Hagihara Y, Matsumura M, Osada S, Kodama H, Higami Y, Fukai F.  Induction of cellular senescence in fibroblasts through β1-integrin activation by tenascin-C-derived peptide and its protumor effect.  Am J Cancer Res  2021  11(9):4364-4379  PubMed ID: 34659892  



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