Cell No. : Cell Name
RCB4466 : HANK1
update : 2024/11/11
|
| Comment | NK/T-cell lymphoma. |
| Comment from the depositor | |
| Terms and conditions | 1)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Br J Haematol 1998 103(3):669-677) designated by the DEPOSITOR is requested.
|
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
KAGAMI, Yoshitoyo
|
| Originator |
KAGAMI, Yoshitoyo
|
| Year of deposit |
2011
|
| Cloning (depositor) |
No
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Age at sampling |
46 years
|
| Tissue |
lymph node
|
| Disease name |
NK/T-cell lymphoma
|
| Tumor |
nasal type NK/T-cell lymphoma, stage IV
|
| Metastatic ability |
Unknown
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Biochemical |
TIA-1(+), Granzyme B(+)
|
| Surface antigen (depositor) |
CD2(+), CD7(+), CD25(+), CD56(+),
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Suspension cells
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
Iscove's Modified Dulbecco's Medium + (20% human plasma ((heparin) or 20% human serum) + 10ng/ml human IL-2
|
IMDM + 20% Human Serum + 10ng/ml human IL-2
|
| Antibiotics |
Free
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Medium + 10% DMSO
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| Virus (EBV) |
(+)
|
(+)
|
| Chromosome mode |
48, XX, +2, del(16q13), +21
|
|
| Immunophenotypic analysis |
|
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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