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Cell No. : Cell Name
RCB4466 : HANK1  update : 2022/05/16
CommentNK/T-cell lymphoma.
Comment from the depositor
Terms and conditions1)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Br J Haematol 1998 103(3):669-677) designated by the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KAGAMI, Yoshitoyo
Originator KAGAMI, Yoshitoyo
Year of deposit 2011
Cloning (depositor) No
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 46 years
Tissue lymph node
Disease name NK/T-cell lymphoma
Tumor nasal type NK/T-cell lymphoma, stage IV
Metastastatic ability Unknown
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Biochemical TIA-1(+), Granzyme B(+)
Surface antigen (depositor) CD2(+), CD7(+), CD25(+), CD56(+),
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives Iscove's Modified Dulbecco's Medium + (20% human plasma ((heparin) or 20% human serum) + 10ng/ml human IL-2 IMDM + 20% Human Serum + 10ng/ml human IL-2
Antibiotics Free Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Virus (EBV) (+) (+)
Chromosome mode 48, XX, +2, del(16q13), +21
Immunophenotypic analysis
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
3354  Y Kagami, S Nakamura, R Suzuki, S Iida, Y Yatabe, Y Okada, T Kobayashi, T Tsurumi, M Seto, M Ogura, O Taguchi, Y Morishima  Establishment of an IL-2-dependent cell line derived from 'nasal-type' NK/T-cell lymphoma of CD2+, sCD3-, CD3epsilon+, CD56+ phenotype and associated with the Epstein-Barr virus  Br J Haematol  1998  103(3):669-77  PubMed ID: 9858215   DOI: 10.1046/j.1365-2141.1998.01029.x

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