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Cell No. : Cell Name
RCB4454 : SR-CDF1DBT update : 2024/11/11 |
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| Comment | Mouse brain tumors induced with Rous sarcom. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures (Jpn J Exp Med 1967 37(5):461-474. Jpn J Exp Med 1970 40(2):79-86. Arch Gesamte Virusforsch 1974 44(3):298-302 ) designated by the DEPOSITOR is requested. 2) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLIGICAL RESOURSE. | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | KUMANISHI, Toshiro | ||
| Originator | YAMANOTO, Tadashi | |||
| Year of deposit | 2011 | |||
| Another name | DBT | |||
| Animal | _mouse < Mammals | |||
| Strain name | BALB/c x DBA/2 | |||
| Gender | Female | |||
| Tissue | subcutaneously passaged mouse brain tumors | |||
| Primary focus | brain | |||
| Tumor | Brain tumor | |||
| SR-RSV-induced brain tumor cells | ||||
| Classification | transformed | Lifespan | infinite | |
| Morphology | fibroblast-like | |||
| Medium | Medium List | Culture type | Adherent cells | Adherent cells |
| Culture medium | 10%FBS+DMEM | DMEM+ 10% FBS | ||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 4 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| SSLP(mouse) | OK | |||
| Reference information | Reference | 3 | ||
| User's Publication | 0 | |||
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