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Cell No. : Cell Name
RCB4454 : SR-CDF1DBT  update : 2024/01/17
CommentMouse brain tumors induced with Rous sarcom.
Comment from the depositor
Terms and conditions1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literatures (Jpn J Exp Med 1967 37(5):461-474. Jpn J Exp Med 1970 40(2):79-86. Arch Gesamte Virusforsch 1974 44(3):298-302 ) designated by the DEPOSITOR is requested. 2) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLIGICAL RESOURSE.
approver's address
Niigata Longevity Research Institute

766 Shimoishikawa, shibata, Niigata 959-2516
Dr.Kumanishi, Toshiro
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor KUMANISHI, Toshiro
Originator YAMANOTO, Tadashi
Year of deposit 2011
Another name DBT
Animal _mouse < Mammals
Strain name BALB/c x DBA/2
Gender Female
Tissue subcutaneously passaged mouse brain tumors
Primary focus brain
Tumor Brain tumor
SR-RSV-induced brain tumor cells
Classification transformed
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium 10%FBS+DMEM DMEM+ 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 3
User's Publication 0

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925  N Hirano, K Fujiwara, S Hino, M Matumoto  Replication and plaque formation of mouse hepatitis virus (MHV-2) in mouse cell line DBT culture  Arch Gesamte Virusforsch  1974  44(3):298-302.  PubMed ID: 4365902   DOI: 10.1007/BF01240618
924  T Kumanishi, T Yamamoto  Brain tumors induced with Rous sarcoma virus, Schmidt-Ruppin strain. 2. Rous tumor specific transplantation antigen in subcutaneously passaged mouse brain tumors  Jpn J Exp Med  1970  40(2):79-86  PubMed ID: 4318037  
923  T Kumanishi  Brain tumors induced with Rous sarcoma virus, Schmidt-Ruppin strain. I. Induction of brain tumors in adult mice with Rous chicken sarcoma cells  Jpn J Exp Med  1967  37(5):461-74.  PubMed ID: 4301953  

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