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Cell No. : Cell Name
RCB4452 : FL-YB  update : 2023/10/16
CommentMouse follicular dendritic cell line
Comment from the depositor
Terms and conditions1) Distribution is limited to users belonging to non-profit organization. 2) Prior permission by the depositor is necessary. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Immunol. 2006;177:5204-5214) designated by the DEPOSITOR is required.
Remarks
approver's address
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English
Address
Okayama University
Graduate School of Natural Science and Technology, Department of Biomedical Engineering
3-1-1 Tsushima-naka, Kita-ku, Okayama-Shi, Okayama-Ken, 700-8530 Japan
Dr.MAGARI Masaki
Fax. +81-86-251-8198
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohmori, Hitoshi
Originator Ohmori, Hitoshi
Year of deposit 2011
Animal _mouse < Mammals
Strain name BALB/c
Genus Mus
Species musculus
Gender Male
Age at sampling adult
Tissue lymph node
Classification transformed
Recombinant recombinant
Exogene mouse BAFF cDNA (pMSCV-BAFF-IRES-enhanced GFP) , puromycin resistance gene
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_6C46
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium (DMEM (low glucose) : RPMI1640 = 1 : 1) + 10% FBS + 10μM 2-Mercaptoethanol + 5ng/ml mouse TNFα
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 0


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Reference
5501  Aungier SR, Ohmori H, Clinton M, Mabbott NA.  MicroRNA-100-5p indirectly modulates the expression of Il6, Ptgs1/2 and Tlr4 mRNA in the mouse follicular dendritic cell-like cell line, FL-Y.  Immunology  2015  144(1):34-44  PubMed ID: 24944008   DOI: 10.1111/imm.12342
5500  Yamane F, Nishikawa Y, Matsui K, Asakura M, Iwasaki E, Watanabe K, Tanimoto H, Sano H, Fujiwara Y, Stanley ER, Kanayama N, Mabbott NA, Magari M, Ohmori H.  CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34.  J Leukoc Biol  2014  95(1):19-31  PubMed ID: 24052571   DOI: 10.1189/jlb.0613311
5894  Masaki Magari, Yumiko Nishikawa, Yasumasa Fujii, Yumi Nishio, Koji Watanabe, Michiya Fujiwara, Naoki Kanayama, Hitoshi Ohmori  IL-21-dependent B cell death driven by prostaglandin E2, a product secreted from follicular dendritic cells  J Immunol  2011  187(8):4210-8  PubMed ID: 21911600   DOI: 10.4049/jimmunol.1100934
5499  Nishikawa Y, Hikida M, Magari M, Kanayama N, Mori M, Kitamura H, Kurosaki T, Ohmori H.  Establishment of lymphotoxin beta receptor signaling-dependent cell lines with follicular dendritic cell phenotypes from mouse lymph nodes.  J Immunol  2006  177(8):5204-14  PubMed ID: 17015706   DOI: 10.4049/jimmunol.177.8.5204

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