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Cell No. : Cell Name
RCB4451 : UPS-1  update : 2023/10/16
CommentSub-line of CHO-K1 which is defective in the non-endocytic uptake of fluorescent analogs of phosphatidylserine (C6-NBD-PS).
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Cell Biol 1995;128:793-804) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Basic information Depositor Hanada, Kentaro
Originator Hanada, Kentaro
Year of deposit 2011
Animal _hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T762
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
deposit info
lot info
Reference information Reference 1
User's Publication 1

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3644  Hanada K, Pagano RE.  A Chinese hamster ovary cell mutant defective in the non-endocytic uptake of fluorescent analogs of phosphatidylserine: isolation using a cytosol acidification protocol.  J Cell Biol  1995  128(5):793-804  PubMed ID: 7876305   DOI: 10.1083/jcb.128.5.793

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User's Publication
15477  Takada N, Takatsu H, Miyano R, Nakayama K, Shin HW.  ATP11C mutation is responsible for the defect in phosphatidylserine uptake in UPS-1 cells  J Lipid Res   2015  56(11):2151-7  PubMed ID: 26420878   DOI: 10.1194/jlr.M062547

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