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Cell No. : Cell Name
RCB4451 : UPS-1  update : 2021/05/20
CommentSub-line of CHO-K1 which is defective in the non-endocytic uptake of fluorescent analogs of phosphatidylserine (C6-NBD-PS).
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Cell Biol 1995;128:793-804) designated by the DEPOSITOR is required. 2) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hanada, Kentaro
Originator Hanada, Kentaro
Year of deposit 2011
Animal _hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T762
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
3644  Hanada K, Pagano RE.  A Chinese hamster ovary cell mutant defective in the non-endocytic uptake of fluorescent analogs of phosphatidylserine: isolation using a cytosol acidification protocol.  J Cell Biol  1995  128(5):793-804  PubMed ID: 7876305

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User's Publication
15477  Takada N, Takatsu H, Miyano R, Nakayama K, Shin HW.  ATP11C mutation is responsible for the defect in phosphatidylserine uptake in UPS-1 cells  J Lipid Res   2015  56(11):2151-7  PubMed ID: 26420878



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