Cell No. : Cell Name
RCB4450 : SR-DDD
update : 2024/11/11
|
| Comment | Ascites sarcoma induced with Rous sarcom. |
| Comment from the depositor | |
| Terms and conditions | 1) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Jpn J Exp Med 1969 39(3):239-51) designated by the DEPOSITOR is requested.
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| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
SHIBUYA, Masabumi
|
| Originator |
TAKEUCHI, Emiko & YAMAMOTO, Tadashi
|
| Year of deposit |
2011
|
| Animal |
_mouse
< Mammals
|
| Strain name |
DDD
|
| Genus |
Mus
|
| Species |
Musculus
|
| Gender |
Unknown
|
| Tissue |
ascites
|
| Tumor |
ascites sarcoma
|
| Genetics |
SR-RSV-induced ascites sarcoma
|
| Classification |
transformed
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Suspension cells
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
10%FBS-F12
|
HamF12 + 10% FBS
|
| Antibiotics |
Kanamycin (100μg/ml)
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
1 : 4-5 split
|
1 : 4 split
|
| SC frequency |
Subculture : 2 times/week
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
Cell Reservoir One (with DMS0)
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| SSLP(mouse) |
|
OK
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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