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Cell No. : Cell Name
RCB4450 : SR-DDD  update : 2022/03/28
CommentAscites sarcoma induced with Rous sarcom.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Jpn J Exp Med 1969 39(3):239-51) designated by the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor SHIBUYA, Masabumi
Originator TAKEUCHI, Emiko & YAMAMOTO, Tadashi
Year of deposit 2011
Animal mouse < Mammals
Strain name DDD
Genus Mus
Species Musculus
Gender Unknown
Tissue ascites
Tumor ascites sarcoma
Genetics SR-RSV-induced ascites sarcoma
Classification transformed
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives 10%FBS-F12 HamF12 + 10% FBS
Antibiotics Kanamycin (100μg/ml) Free
Passage method dilution
Culture information Passage ratio 1 : 4-5 split 1 : 4 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Cell Reservoir One (with DMS0) Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 1
User's Publication 0


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Reference
7460  Takeuchi M, Hino S, Yamamoto T.  Studies on rous sarcoma virus in mice. 3. Three lines of SR-RSV-induced mouse ascites sarcoma.  Jpn J Exp Med  1969  39(3):239-51  PubMed ID: 4312682  

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