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Cell No. : Cell Name
RCB4449 : SR-C3H/He  update : 2022/03/28
CommentAscites sarcoma induced with Rous sarcom.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Jpn J Exp Med 1967. 37(1):37-50) designated by the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor SHIBUYA, Masabumi
Originator YAMAMOTO, Tadashi
Year of deposit 2011
Animal mouse < Mammals
Strain name C3H/He
Genus Mus
Species Musculus
Gender Unknown
Tissue ascites
Tumor ascites sarcoma
Genetics SR-RSV-induced ascites sarcoma
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives 10%FBS-DMEM DMEM + 10% FBS
Antibiotics Kanamycin (100μg/ml) Free
Passage method dilution
Culture information Passage ratio 1 : 4-5 split 1 : 4 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Cell Reservoir One (with DMS0) Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 1
User's Publication 0


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Reference
7457  Yamamoto T, Takeuchi M.  Studies on Rous sarcoma virus in mice. I. Establishment of an ascites sarcoma induced by the Schmidt-Ruppin strain of Rous sarcoma virus in C3H-He mouse.  Jpn J Exp Med  1967.  37(1):37-50  PubMed ID: 4293253  

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