Back
|
Cell No. : Cell Name
RCB4393 : SETDB-1(Z4606) update : 2024/11/11 |
||||
| Comment | Hybridoma against SETDB-1 : SET domain, bifurcated 1 (1025-1080(56aa)) antigen. | |||
| Comment from the depositor | SET domain, bifurcated 1 (1025-1080(56aa)) was expressed on the surface of baculovirus as the gp64 fusion protein according to the previously described procedure (J Atheroscler Thromb. 2002;9:233-242). | |||
| Terms and conditions | 1) Publishing the works using these antibodies, please acknowledge that their production was supported by the project of "Development of New Functional Antibody Technologies of the New Energy and Industrial Technology Development Organization (NEDO)". 2) No recipient shall use the research material, their progeny or derivatives, commercially without depositor's prior written approval. | |||
| Remarks | ||||
| approver's address |
×
|
|||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | HAMAKUBO, Takao | ||
| Originator | HAMAKUBO, Takao | |||
| Year of deposit | 2011 | |||
| Animal | _mouse x mouse < Hybrid | |||
| Tissue | hybridoma | |||
| Classification | hybrid cells | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_C5Z4 | hybridoma | Fusion_cell_1 name | NS-1 |
| Fusion_cell_1 strain | mouse | |||
| Fusion_cell_2 name | spleen | |||
| Fusion_cell_2 strain | mouse, BALB/c | Fusion method | PEG, HAT selection | Antibody name | Z4606 |
| Subclass | IgG2a(Kappa) | |||
| Immunogen | Gp64 fusion SET domain, bifurcated 1 (1025-1080(56aa)) expressed on the budded baculovirus. | |||
| Medium | Medium List | Culture type | Suspension cells | Suspension cells |
| Culture method | 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | (RPMI1640+1mM Sodium pyruvate+146μg/ml L-Glutamine)+15%FBS) : (S-Clone CM-B) = 3 : 1 . Thawing medium : Medium + 10% BM-Condimed H1. | (RPMI1640+1mM Sodium pyruvate+146μg/ml L-Glutamine)+15%FBS) : (S-Clone CM-B) = 3 : 1 . Thawing medium : Medium + 10% BM-Condimed H1. | ||
| Antibiotics | Free | Passage method | dilution | dilution | Culture information | Passage cell No | 0.1-1x10 5 cells/ml |
| Passage ratio | 1 : 10-20 split | 1 : 10-20 split | ||
| SC frequency | Subculture : 2 times/week | Subculture : 2-3 times/week | ||
| Temperature | 37 ℃ | 37 ℃ | ||
| CO2 concentration | 5 % | 5 % | ||
| Freeze medium | CELLBANKER (Juji-field BLC-1) | CELLBANKER 1 | ||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Mycoplasma | (-) | |||
| Antibody subclass | IgG2a | IgG2a(Kappa) | ||
| Saturation density | 2-3x10 5 cells/ml | |||
| Doubling time | 24 hr | |||
| Others | If cultured for a long period of time, the antibody titer may decrease. | |||
| Reference information | Reference | 1 | ||
| User's Publication | 0 | |||
Top
Top
| User's Publication |
Back
CELL BANK Website