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Cell No. : Cell Name
RCB4212 : Prdm16-2(Z1301)  update : 2023/03/27
CommentHybridoma against Prdm16-2 : PR domain containing 16 (467-566(100aa)) antigen.
Comment from the depositorPR domain containing 16 (467-566(100aa)) was expressed on the surface of baculovirus as the gp64 fusion protein according to the previously described procedure (J Atheroscler Thromb. 2002;9:233-242).
Terms and conditions1) Publishing the works using these antibodies, please acknowledge that their production was supported by the project of "Development of New Functional Antibody Technologies of the New Energy and Industrial Technology Development Organization (NEDO)". 2) No recipient shall use the research material, their progeny or derivatives, commercially without depositor's prior written approval.
approver's address
3rd Fl., Sangakurenkei-Plaza, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Fax. +81-3-5805-7699
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
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Basic information Depositor HAMAKUBO, Takao
Originator HAMAKUBO, Takao
Year of deposit 2011
Animal _mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
hybridoma Fusion_cell_1 name NS-1
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name Z1301
Subclass IgG2a(Kappa)
Immunogen Gp64 fusion PR domain containing 16 (467-566(100aa)) expressed on the budded baculovirus. 
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium (RPMI1640+1mM Sodium pyruvate+146μg/ml L-Glutamine)+15%FBS) : (S-Clone CM-B) = 3 : 1 . Thawing medium : Medium + 10% BM-Condimed H1.
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10-20 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a(Kappa)
deposit info
lot info
Reference information Reference 0
User's Publication 0

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