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Cell No. : Cell Name
RCB4081 : Cys-SN2(N3312)  update : 2022/01/20
CommentHybridoma against Cys-SN2 :Cystatin (92-117 (26aa)) antigen.
Comment from the depositorCystatin(92-117(26aa)) was expressed on the surface of baculovirus as the gp64 fusion protein according to the previously described procedure (J Atheroscler Thromb. 2002;9:233-242).
Terms and conditions1) Publishing the works using these antibodies, please acknowledge that their production was supported by the project of "Development of New Functional Antibody Technologies of the New Energy and Industrial Technology Development Organization (NEDO)". 2) No recipient shall use the research material, their progeny or derivatives, commercially without depositor's prior written approval.
Remarks
approver's address
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Address
3rd Fl., Sangakurenkei-Plaza, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
TODAI TLO, Ltd.
shigeta@todaitlo.jp
Fax. +81-3-5805-7699
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor HAMAKUBO, Takao
Originator HAMAKUBO, Takao
Year of deposit 2011
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_A6XW
hybridoma Fusion_cell_1 name NS-1
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name N3312
Subclass IgG2a
Immunogen Gp64 fusion Cystatin(92-117(26aa)) expressed on the budded baculovirus.
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives (RPMI1640+1mM Sodium pyruvate+146μg/ml L-Glutamine)+15%FBS) : (S-Clone CM-B) = 3 : 1
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a(Kappa)
Others Thawing medium : Medium + 10% BM-Condimed H1
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Reference information Reference 0
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