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Cell No. : Cell Name
RCB3708 : Atg3 +/+ MEFs  update : 2021/05/20
CommentMouse embryonic fibroblast cell line derived from wild type C57BL/6 strain.
Comment from the depositor
Terms and conditions1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the approver. 2) The RECIPIENT must contact the DEVELOPER in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Mol Biol Cell. 2008;19:4762-75) designated by the DEPOSITOR is required. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 5) The RECIPIENT of BIOLOGICAL RESOURCE who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR.
approver's address
Juntendo University Graduate School of Medicine
Department of Physiology
2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421 Japan
Dr.KOMATSU Masaaki
Fax. +81-3-3813-1609
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Komatsu, Masaaki
Originator Komatsu, Masaaki
Year of deposit 2011
Animal mouse < Mammals
Strain name C57BL/6
Genus mus
Species musculus
Gender Unknown
Age at sampling 2 weeks embryo
Tissue embryo/fetus, whole
Classification transformed
Recombinant recombinant
Exogene SV40 large T, pMESVts, Neomycin resistance gene
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_1T21
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose, without Sodium Pyruvate) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
deposit info
lot info
Reference information Reference 1
User's Publication 0

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3643  Sou YS, Waguri S, Iwata J, Ueno T, Fujimura T, Hara T, Sawada N, Yamada A, Mizushima N, Uchiyama Y, Kominami E, Tanaka K, Komatsu M.  The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice.  Mol Biol Cell  2008  19(11):4762-75  PubMed ID: 18768753   DOI: 10.1091/mbc.e08-03-0309

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