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Cell No. : Cell Name
RCB3706 : Atg7 +/+ MEFs  update : 2024/07/11
CommentMouse embryonic fibroblasts derived from wild type mouse (C57BL/6).
Comment from the depositor
Terms and conditions1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the approver. 2) The RECIPIENT must contact the DEVELOPER in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Cell Biol 2005;169:425-34) designated by the DEPOSITOR is required. 4) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.5) The RECIPIENT of BIOLOGICAL RESOURCE who belongs to a profit organization must obtain a prior written consent on use of it from the DEPOSITOR.
approver's address
Juntendo University Graduate School of Medicine
Department of Physiology
2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421 Japan
Dr.KOMATSU Masaaki
Fax. +81-3-3813-1609
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Komatsu, Masaaki
Originator Komatsu, Masaaki
Year of deposit 2011
Animal _mouse < Mammals
Strain name C57BL/6
Gender Unknown
Age at sampling embryo
Tissue embryo/fetus, whole
Classification semi-normal
Recombinant recombinant
Exogene SV40 large T, pMESVts, Neomycin resistance gene
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_VQ60
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (high glucose, without Sodium Pyruvate) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
SSLP(mouse) OK
Reference information Reference 1
User's Publication 4

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3642  Komatsu M, Waguri S, Ueno T, Iwata J, Murata S, Tanida I, Ezaki J, Mizushima N, Ohsumi Y, Uchiyama Y, Kominami E, Tanaka K, Chiba T.  Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice..  J Cell Biol  2005  169(3):425-34  PubMed ID: 15866887   DOI: 10.1083/jcb.200412022

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User's Publication
16981  Uchida Y, Torisu K, Ueki K, Tsuruya K, Nakano T, Kitazono T.  Autophagy gene ATG7 regulates albumin transcytosis in renal tubule epithelial cells  Am J Physiol Renal Physiol  2021  321(5):F572-F586  PubMed ID: 34541900   DOI: 10.1152/ajprenal.00172.2021
10410  Stephanie U. Greer, Margret H. Ogmundsdottir, Jiamin Chen, Billy T. Lau, Richard Glenn C. Delacruz, Imelda T. Sandoval, Sigrun Kristjansdottir, David A. Jones, Derrick S. Haslem, Robin Romero, Gail Fulde, John M. Bell, Jon G. Jonasson, Eirikur Steingrimsson, Hanlee P. Ji, Lincoln D. Nadauld  Genetic risk of cholangiocarcinoma is linked to the autophagy gene ATG7  bioRxiv  2019  November 22    DOI: 10.1101/836767
4149  Sawai H.  Desipramine-induced lysosomal vacuolization is independent of autophagy.  Cell Biol. Int.  2018    PubMed ID: 29068103   DOI: 10.1002/cbin.10901
9173  Alexa R Wilden, Joshua A Molina, Melissa Feuerborn, Daniel Boyle, Stella Y Lee  Glutamine-dependent Lysosome Homeostatic Changes Induced by Starvation and Lysosome Inhibition  Biochim Biophys Acta Mol Cell Res  2018  1865 (9), 1356-1367  PubMed ID: 29966622   DOI: 10.1016/j.bbamcr.2018.06.014

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