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Cell No. : Cell Name
RCB3686 : THP-1  update : 2023/04/21
CommentHuman cell line derived from monocytic leukemia. TKG0267 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Tsuchiya, S.
Year of deposit 2011
Original cell TKG0267
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 1 year
Tissue peripheral blood
Disease name acute monocytic leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0267)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0006
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
STR(human) OK
deposit info
lot info
Reference information Reference 4
User's Publication 6

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4951  Tsuchiya S, Minegishi N, Minegishi M, Sato T, Konno T.  Adaptation of a human monocytic leukemia cell line (THP-1) in a protein-free chemically defined medium.  Tohoku J Exp Med  1986  150(4):455-65  PubMed ID: 3551191   DOI: 10.1620/tjem.150.455
4950  Tajima S, Hayashi R, Tsuchiya S, Miyake Y, Yamamoto A.  Cells of a human monocytic leukemia cell line (THP-1) synthesize and secrete apolipoprotein E and lipoprotein lipase.  Biochem Biophys Res Commun  1985  126(1):526-31  PubMed ID: 3855620   DOI: 10.1016/0006-291x(85)90637-0
4949  Tsuchiya S, Kobayashi Y, Goto Y, Okumura H, Nakae S, Konno T, Tada K.  Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester.  Cancer Res  1982  42(4):1530-6  PubMed ID: 6949641  
720  Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K.  Establishment and characterization of a human acute monocytic leukemia cell line (THP-1).  Int J Cancer  1980  26(2):171-6  PubMed ID: 6970727   DOI: 10.1002/ijc.2910260208

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User's Publication
21183  Xiao L, Mochizuki M, Wang D, Shimamura N, Sunada K, Nakahara T.  Types of cell culture inserts affect cell crosstalk between co-cultured macrophages and adipocytes.  Biochem Biophys Res Commun  2023  658:10-17  PubMed ID: 37011478   DOI: 10.1016/j.bbrc.2023.03.068
21221  Fujita H, Adachi C, Inoue T.  Cholesterol-load evokes robust calcium response in macrophages: An early event toward cholesterol-induced macrophage death.  Cell Calcium  2023  113:102754  PubMed ID: 37196488   DOI: 10.1016/j.ceca.2023.102754
20628  Xiao L, Mochizuki M, Fan Y, Nakahara T, Liao F.  Enzyme-digested Colla Corii Asini (E'jiao) suppresses lipopolysaccharide-induced inflammatory changes in THP-1 macrophages and OP9 adipocytes.  Hum Cell  2022    PubMed ID: 35359251   DOI: 10.1007/s13577-022-00694-5
20834  Mori T, Kataoka H, Tanabe G, Into T.  Solubility affects IL-1β-producing activity of the synthetic candidalysin peptide.  PLoS One  2022  17(8):e0273663  PubMed ID: 36040970   DOI: 10.1371/journal.pone.0273663
20361  Okamoto Y, Kitakaze K, Takenouchi Y, Yamamoto S, Ishimaru H, Tsuboi K.  Sphingosine 1-phosphate receptor type 2 positively regulates interleukin (IL)-4/IL-13-induced STAT6 phosphorylation.  Cell Signal  2021  88:110156  PubMed ID: 34592416   DOI: 10.1016/j.cellsig.2021.110156
13052  Kawai S, Suzuki M, Arimoto S, Korenaga T, Yasukawa T.  Determination of membrane capacitance and cytoplasm conductivity by simultaneous electrorotation.  Analyst  2020    PubMed ID: 32462157   DOI: 10.1039/d0an00100g

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