Cell No. : Cell Name
RCB3686 : THP-1
update : 2023/04/21
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| Comment | Human cell line derived from monocytic leukemia. TKG0267 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Fukuda, Hiroshi
|
| Originator |
Tsuchiya, S.
|
| Year of deposit |
2011
|
| Original cell |
TKG0267
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
1 year
|
| Tissue |
peripheral blood
|
| Disease name |
acute monocytic leukemia
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0267）
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|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_0006
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture medium |
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RPMI1640 + 10% FBS
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| Antibiotics |
|
Free
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|
Passage method |
|
dilution
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
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Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
(-)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
6
|
| Reference |
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4951
Tsuchiya S, Minegishi N, Minegishi M, Sato T, Konno T.
Adaptation of a human monocytic leukemia cell line (THP-1) in a protein-free chemically defined medium.
Tohoku J Exp Med
1986
150(4):455-65
PubMed ID: 3551191
DOI: 10.1620/tjem.150.455
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4950
Tajima S, Hayashi R, Tsuchiya S, Miyake Y, Yamamoto A.
Cells of a human monocytic leukemia cell line (THP-1) synthesize and secrete apolipoprotein E and lipoprotein lipase.
Biochem Biophys Res Commun
1985
126(1):526-31
PubMed ID: 3855620
DOI: 10.1016/0006-291x(85)90637-0
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4949
Tsuchiya S, Kobayashi Y, Goto Y, Okumura H, Nakae S, Konno T, Tada K.
Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester.
Cancer Res
1982
42(4):1530-6
PubMed ID: 6949641
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720
Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K.
Establishment and characterization of a human acute monocytic leukemia cell line (THP-1).
Int J Cancer
1980
26(2):171-6
PubMed ID: 6970727
DOI: 10.1002/ijc.2910260208
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| User's Publication |
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21183
Xiao L, Mochizuki M, Wang D, Shimamura N, Sunada K, Nakahara T.
Types of cell culture inserts affect cell crosstalk between co-cultured macrophages and adipocytes.
Biochem Biophys Res Commun
2023
658:10-17
PubMed ID: 37011478
DOI: 10.1016/j.bbrc.2023.03.068
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21221
Fujita H, Adachi C, Inoue T.
Cholesterol-load evokes robust calcium response in macrophages: An early event toward cholesterol-induced macrophage death.
Cell Calcium
2023
113:102754
PubMed ID: 37196488
DOI: 10.1016/j.ceca.2023.102754
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20628
Xiao L, Mochizuki M, Fan Y, Nakahara T, Liao F.
Enzyme-digested Colla Corii Asini (E'jiao) suppresses lipopolysaccharide-induced inflammatory changes in THP-1 macrophages and OP9 adipocytes.
Hum Cell
2022
PubMed ID: 35359251
DOI: 10.1007/s13577-022-00694-5
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20834
Mori T, Kataoka H, Tanabe G, Into T.
Solubility affects IL-1β-producing activity of the synthetic candidalysin peptide.
PLoS One
2022
17(8):e0273663
PubMed ID: 36040970
DOI: 10.1371/journal.pone.0273663
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20361
Okamoto Y, Kitakaze K, Takenouchi Y, Yamamoto S, Ishimaru H, Tsuboi K.
Sphingosine 1-phosphate receptor type 2 positively regulates interleukin (IL)-4/IL-13-induced STAT6 phosphorylation.
Cell Signal
2021
88:110156
PubMed ID: 34592416
DOI: 10.1016/j.cellsig.2021.110156
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13052
Kawai S, Suzuki M, Arimoto S, Korenaga T, Yasukawa T.
Determination of membrane capacitance and cytoplasm conductivity by simultaneous electrorotation.
Analyst
2020
PubMed ID: 32462157
DOI: 10.1039/d0an00100g
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