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Cell No. : Cell Name
RCB3685 : HMV-II  update : 2022/11/25
CommentHuman cell line derived from melanotic melanoma (highly melanotic). TKG0318 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Kasuga, T.
Year of deposit 2011
Original cell TKG0318
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 65 years
Tissue vagina
Disease name melanoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0318)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_1282
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
deposit info
lot info
Reference information Reference 3
User's Publication 0

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1534  Inoue S, Ito S, Imai Y, Kasuga T, Fujita K.  Growth inhibition of melanoma cells by N-protected dopa derivatives.  Biochem Pharmacol  1987  36(20):3537-40  PubMed ID: 3118879   DOI: 10.1016/0006-2952(87)90340-6
1536  Yamada I, Seki S, Matsubara O, Ito S, Suzuki S, Kasuga T.  The cytotoxicity of cysteinylcatechols and related compounds to human melanoma cells in vitro.  J Invest Dermatol  1987  88(5):538-40  PubMed ID: 3572026   DOI: 10.1111/1523-1747.ep12470098
968  Hasumi K, Sakamoto G, Sugano H, Kasuga T, Masubuchi K  Primary malignant melanoma of the vagina: study of four autopsy cases with ultrastructural findings.  Cancer  1978  42:2675-86  PubMed ID: 365316   DOI: 10.1002/1097-0142(197812)42:6<2675::aid-cncr2820420624>;2-g

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