Cell No. : Cell Name
RCB3685 : HMV-II
update : 2023/10/16
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| Comment | Human cell line derived from melanotic melanoma (highly melanotic). TKG0318 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Fukuda, Hiroshi
|
| Originator |
Kasuga, T.
|
| Year of deposit |
2011
|
| Original cell |
TKG0318
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
65 years
|
| Tissue |
vagina
|
| Disease name |
melanoma
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0318）
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|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_1282
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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HamF12 + 10% FBS
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| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 4 split
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| SC frequency |
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Subculture : once/week, Medium Renewal : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
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| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma |
|
(-)
|
| Animal PCR |
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OK
|
| STR(human) |
|
OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
1534
Inoue S, Ito S, Imai Y, Kasuga T, Fujita K.
Growth inhibition of melanoma cells by N-protected dopa derivatives.
Biochem Pharmacol
1987
36(20):3537-40
PubMed ID: 3118879
DOI: 10.1016/0006-2952(87)90340-6
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1536
Yamada I, Seki S, Matsubara O, Ito S, Suzuki S, Kasuga T.
The cytotoxicity of cysteinylcatechols and related compounds to human melanoma cells in vitro.
J Invest Dermatol
1987
88(5):538-40
PubMed ID: 3572026
DOI: 10.1111/1523-1747.ep12470098
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968
Hasumi K, Sakamoto G, Sugano H, Kasuga T, Masubuchi K
Primary malignant melanoma of the vagina: study of four autopsy cases with ultrastructural findings.
Cancer
1978
42:2675-86
PubMed ID: 365316
DOI: 10.1002/1097-0142(197812)42:6<2675::aid-cncr2820420624>3.0.co;2-g
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