Cell No. : Cell Name
RCB3682 : HeLa S3
update : 2023/10/16
|
| Comment | Human cell line derived from cervical cancer. TKG0444 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Fukuda, Hiroshi
|
| Originator |
T.T. Puck.
|
| Year of deposit |
2011
|
| Original cell |
TKG0444
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
31 years
|
| Tissue |
cervical carcinoma
|
| Classification |
cancer
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0444）
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_0058
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
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|
Passage method |
|
0.02% EDTA-PBS or pipetting
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Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
6
|
| User's Publication |
0
|
| Reference |
|
207
Prescott,D.M. (ed.)
Methods Cell Biol
1973
4:1-42
|
|
350
Kuroki T.
Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.
Exp Cell Res
1973
80(1):55-62
PubMed ID: 4361348
DOI: 10.1016/0014-4827(73)90274-7
|
|
1446
J E DARNELL Jr, T K SAWYERc
Variation in plaque-forming ability among parental and clonal strains of HeLa cells
Virology
1959
223-9
PubMed ID: 13669339
DOI: 10.1016/0042-6822(59)90006-6
|
|
1445
SATO G, FISHER HW, PUCK TT.
Molecular growth requirements of single mammalian cells.
Science
1957
126(3280):461-4
PubMed ID: 13486039
|
|
206
PUCK TT, MARCUS PI, CIECIURA SJ.
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
J Exp Med
1956
103(2):273-83
PubMed ID: 13286432
DOI: 10.1084/jem.103.2.273
|
|
150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
|
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