Cell No. : Cell Name RCB3680 : HeLa  update : 2024/09/05
Comment			Human cell line derived from cervical cancer. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cycline and MC210. TKG0331 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Fukuda, Hiroshi
		Originator	Gey, G. O.
		Year of deposit	2011
		Original cell	TKG0331
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Female
		Age at sampling	31 years
		Tissue	cervical carcinoma
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0331）
		Lifespan	infinite
		Morphology	epithelial-like
		Cellosaurus(Expasy)	CVCL_0030
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8-10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	7

Top

Reference

1446  J E DARNELL Jr, T K SAWYERc  Variation in plaque-forming ability among parental and clonal strains of HeLa cells  Virology  1959  223-9  PubMed ID: 13669339   DOI: 10.1016/0042-6822(59)90006-6

1444  Puck TT1, Marcus PI.  A RAPID METHOD FOR VIABLE CELL TITRATION AND CLONE PRODUCTION WITH HELA CELLS IN TISSUE CULTURE: THE USE OF X-IRRADIATED CELLS TO SUPPLY CONDITIONING FACTORS.  Proc Natl Acad Sci U S A  1955  41(7):432-7  PubMed ID: 16589695   DOI: 10.1073/pnas.41.7.432

150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265

Top

User's Publication

22220  Takeda H, Matsuzawa Y, Takeuchi M, Takahashi M, Nishida K, Harayama T, Todoroki Y, Shimizu K, Sakamoto N, Oka T, Maekawa M, Chung MH, Kurizaki Y, Kiuchi S, Tokiyoshi K, Buyantogtokh B, Kurata M, Kvasnička A, Takeda U, Uchino H, Hasegawa M, Miyamoto J, Tanabe K, Takeda S, Mori T, Kumakubo R, Tanaka T, Yoshino T, Okamoto M, Takahashi H, Arita M, Tsugawa H.  MS-DIAL 5 multimodal mass spectrometry data mining unveils lipidome complexities.  Nat Commun  2024  15(1):9903  PubMed ID: 39609386   DOI: 10.1038/s41467-024-54137-w

17800  Asano-Inami E, Yokoi A, Sugiyama M, Hyodo T, Hamaguchi T, Kajiyama H.  The association of UBAP2L and G3BP1 mediated by small nucleolar RNA is essential for stress granule formation  Commun Biol  2023  6(1):415  PubMed ID: 37059803   DOI: 10.1038/s42003-023-04754-w

11381  Win NN, Kyaw MM, Prema, Ngwe H, Ito T, Asakawa Y, Okamoto Y, Tanaka M, Abe I, Morita H.  Dinorcassane Diterpenoid from Boesenbergia rotunda Rhizomes Collected in Lower Myanmar.  Chem. Biodivers.  2019    PubMed ID: 30645035   DOI: 10.1002/cbdv.201800657

12436  Ozeki K, Sugiyama M, Akter KA, Nishiwaki K, Asano-Inami E, Senga T.  FAM98A is localized to stress granules and associates with multiple stress granule-localized proteins  Mol Cell Biochem  2019  451(1-2):107-115  PubMed ID: 29992460   DOI: 10.1007/s11010-018-3397-6

9988  Li S, Gao A, Dong S, Chen Y, Sun S, Lei Z, Zhang Z.  Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.  Int. J. Biol. Macromol.  2017  96:26-34  PubMed ID: 27939510   DOI: 10.1016/j.ijbiomac.2016.12.007

9792  Hyodo T, Ito S, Asano-Inami E, Chen D, Senga T.  A regulatory subunit of protein phosphatase 2A, PPP2R5E, regulates the abundance of microtubule crosslinking factor 1.  FEBS J.  2016  283:3662-3671  PubMed ID: 27521566   DOI: 10.1111/febs.13835

15027  Dan Zhu, Hongyi Sun, Shuhong Li, Xuansheng Hu, Xi Yuan, Chao Han, Zhenya Zhang  Influence of Drying Methods on Antioxidant Activities and Immunomodulatory of Aqueous Extract From Soybean Curd Residue Fermentated by Grifola frondosa  International Journal of Biology  2014  7:82-92    DOI: 10.5539/ijb.v7n1p82