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Cell No. : Cell Name
RCB3678 : BeWo  update : 2023/10/16
CommentHuman cell line derived from choriocarcinoma. TKG0567 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Pattillo, R.A. and Gey, G.O.
Year of deposit 2011
Original cell TKG0567
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue fetal placenta
Disease name choriocarcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0567)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0044
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 15% FBS
Antibiotics Free
Passage method 0.02% EDTA-PBS
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Mycoplasma/Acholeplasma (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 5
User's Publication 2


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Reference
2756  Sheppard DM, Fisher RA, Lawler SD.  Karyotypic analysis and chromosome polymorphisms in four choriocarcinoma cell lines.  Cancer Genet Cytogenet  1985  16(3):251-8  PubMed ID: 4038916   DOI: 10.1016/0165-4608(85)90052-4
2755  Sasaki S, Katayama PK, Roesler M, Pattillo RA, Mattingly RF, Ohkawa K.  Cytogenetic analysis of choriocarcinoma cell lines.  Nihon Sanka Fujinka Gakkai Zasshi  1982  34(12):2253-6  PubMed ID: 7153601  
2680  Pattillo RA, Gey GO.  The establishment of a cell line of human hormone-synthesizing trophoblastic cells in vitro.  Cancer Res  1968  28(7):1231-6  PubMed ID: 4299001  
4980  Pattillo RA, Gey GO, Delfs E, Mattingly RF.  Human hormone production in vitro.  Science  1968  159(3822):1467-9  PubMed ID: 5753554   DOI: 10.1126/science.159.3822.1467
4979  HERTZ R.  Choriocarcinoma of women maintained in serial passage in hamster and rat.  Proc Soc Exp Biol Med  1959  102:77-81  PubMed ID: 14401422   DOI: 10.3181/00379727-102-25149

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User's Publication
17540  Yuki Ochiai, Chigure Suzuki, Katsumori Segawa, Shigekazu Nagata  Inefficient development of syncytiotrophoblasts in the Atp11a-deficient mouse placenta  Proc Natl Acad Sci U S A  2022  119(18):e2200582119  PubMed ID: 35476530   DOI: 10.1073/pnas.2200582119
4195  Ishigaki Y, Sato K.  Effects of Microchannel Shape and Ultrasonic Mixing on Microfluidic Padlock Probe Rolling Circle Amplification (RCA) Reactions.  Micromachines (Basel)  2018    PubMed ID: 30424205   DOI: 10.3390/mi9060272



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