細胞番号 : 細胞名
RCB3677 : A549
update : 2024/01/05
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| 細胞特性(Comment:英) | Human cell line derived from lung cancer. Epithelial-like cells. HLA-A 2603/3001. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cycline and MC210. TKG0184 (Deposited from Tohoku Univ.). |
| 細胞特性(日) | ヒト肺癌由来細胞株。上皮様細胞。 TKG0184 (東北大学医用細胞資源センターからの寄託)。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Fukuda, Hiroshi
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| 樹立者 |
Giard, D. J. et al.
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| 寄託日 |
2011
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| 元の細胞 |
TKG0184
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 性別 |
Male
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| 採取組織 |
lung
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| 病名 |
adenocarcinoma
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| 細胞分類 |
cancer
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| 入手歴 |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0184）
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|
細胞寿命 |
infinite
|
| 細胞形態 |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_0023
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細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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| 培地 |
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MEM + 10% FBS + 0.1mM NEAA
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| 抗生物質 |
|
Free
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|
継代方法 |
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0.25% Trypsin
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|
継代密度 |
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1 : 8-10 split
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| 継代・培地交換頻度 |
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Subculture : 2 times/week
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| 培養最適温度 |
|
37
℃
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| 二酸化炭素濃度 |
|
5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ |
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(-)
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| 動物種PCR |
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OK
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| 個体識別検査 |
|
OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
2件
|
| Reference(日) |
1件
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| 利用者成果(英) |
13件
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| 利用者成果(日) |
0件
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| 利用者成果(英) |
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21652
Yoshida T, Goto K, Kodama A, Bolidong D, Seto T, Hanayama R.
Extracellular vesicles promote silica nanoparticle aggregation that inhibits silica-induced cytotoxicity.
Arch Biochem Biophys
2024
755:109964
PubMed ID: 38527699
DOI: 10.1016/j.abb.2024.109964
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17744
Kawasaki S, Ono H, Hirosawa M, Kuwabara T, Sumi S, Lee S, Woltjen K, Saito H.
Programmable mammalian translational modulators by CRISPR-associated proteins
Nat Commun
2023
14(1):2243
PubMed ID: 37076490
DOI: 10.1038/s41467-023-37540-7
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19947
Moriizumi H, Kubota Y, Tsuchiya T, Naka R, Takekawa M.
Caspase 3-specific cleavage of MEK1 suppresses ERK signaling and sensitizes cells to stress-induced apoptosis
FEBS Open Bio
2023
13(4):684-700
PubMed ID: 36776127
DOI: 10.1002/2211-5463.13574
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21118
Aki T, Kukita M, Takata M, Funakoshi T, Unuma K, Uemura K.
Induction of filamin-C and its involvement in the regulation of cellular senescence and apoptosis in Huh-7 hepatoma cells during arsenic trioxide exposure.
Biochem Biophys Res Commun
2023
651:92-97
PubMed ID: 36801614
DOI: 10.1016/j.bbrc.2023.02.020
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16391
Sakamoto T, Yu Z, Otani Y.
Dual-Color Fluorescence Switch-On Probe for Imaging G-Quadruplex and Double-Stranded DNA in Living Cells
Anal Chem
2022
Mar 2
PubMed ID: 35234461
DOI: 10.1021/acs.analchem.1c04804
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20771
Kobayashi-Sakamoto M, Maeda T, Kimura M, Yusa J, Ito H, Tani H, Kato Y, Hirose K.
Bovine lactoferrin increases the poly(I:C)-induced antiviral response in vitro.
Biochem Cell Biol
2022
PubMed ID: 35830725
DOI: 10.1139/bcb-2021-0342
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20774
Kubota Y, Fujioka Y, Patil A, Takagi Y, Matsubara D, Iijima M, Momose I, Naka R, Nakai K, Noda NN, Takekawa M.
Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.
Nat Commun
2022
13(1):4063
PubMed ID: 35831322
DOI: 10.1038/s41467-022-31690-w
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20891
Matsuya Y, Kai T, Parisi A, Yoshii Y, Sato T.
Application of a simple DNA damage model developed for electrons to proton irradiation.
Phys Med Biol
2022
PubMed ID: 36228611
DOI: 10.1088/1361-6560/ac9a20
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20954
Ohe S, Kubota Y, Yamaguchi K, Takagi Y, Nashimoto J, Kozuka-Hata H, Oyama M, Furukawa Y, Takekawa M.
ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II.
Nat Commun
2022
13(1):7476
PubMed ID: 36463234
DOI: 10.1038/s41467-022-35230-4
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20360
Kashio-Yokota Y, Sato S, Hirose T, Watanabe T, Endo A, Watanabe F, Endo M, Ohba K, Mori T, Takahashi K.
Elevated (Pro)renin Receptor Expression by Anti-Cancer Drugs, Carboplatin and Paclitaxel, in Cultured Cancer Cells: Possible Involvement of Apoptosis and Autophagy.
Tohoku J Exp Med
2021
255(2):91-104
PubMed ID: 34645770
DOI: 10.1620/tjem.255.91
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20756
Xie Y, Wang B, Du L, Wang Y, Xu C, Zhang H, Wen K, Liu Q, Katsube T.
ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines.
Cancer Biol Med
2021
PubMed ID: 34546667
DOI: 10.20892/j.issn.2095-3941.2020.0569
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13571
Melanny Ika Sulistyowaty et al
Synthesis, In Vitro Anticancer Activity and In Silico Study of some Benzylidene Hydrazide Derivatives
Key Engineering Materials
2020
840:277-283
DOI: 10.4028/www.scientific.net/KEM.840.277
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11381
Win NN, Kyaw MM, Prema, Ngwe H, Ito T, Asakawa Y, Okamoto Y, Tanaka M, Abe I, Morita H.
Dinorcassane Diterpenoid from Boesenbergia rotunda Rhizomes Collected in Lower Myanmar.
Chem. Biodivers.
2019
PubMed ID: 30645035
DOI: 10.1002/cbdv.201800657
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