Cell No. : Cell Name
RCB3677 : A549
update : 2024/08/14
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| Comment | Human cell line derived from lung cancer. Epithelial-like cells. HLA-A 2603/3001. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cycline and MC210. TKG0184 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Fukuda, Hiroshi
|
| Originator |
Giard, D. J. et al.
|
| Year of deposit |
2011
|
| Original cell |
TKG0184
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
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| Species |
sapiens
|
| Gender |
Male
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| Tissue |
lung
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| Disease name |
adenocarcinoma
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| Classification |
cancer
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| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0184）
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|
Lifespan |
infinite
|
| Morphology |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_0023
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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MEM + 10% FBS + 0.1mM NEAA
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8-10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
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| User's Publication |
12
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| User's Publication |
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17744
Kawasaki S, Ono H, Hirosawa M, Kuwabara T, Sumi S, Lee S, Woltjen K, Saito H.
Programmable mammalian translational modulators by CRISPR-associated proteins
Nat Commun
2023
14(1):2243
PubMed ID: 37076490
DOI: 10.1038/s41467-023-37540-7
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19947
Moriizumi H, Kubota Y, Tsuchiya T, Naka R, Takekawa M.
Caspase 3-specific cleavage of MEK1 suppresses ERK signaling and sensitizes cells to stress-induced apoptosis
FEBS Open Bio
2023
13(4):684-700
PubMed ID: 36776127
DOI: 10.1002/2211-5463.13574
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21118
Aki T, Kukita M, Takata M, Funakoshi T, Unuma K, Uemura K.
Induction of filamin-C and its involvement in the regulation of cellular senescence and apoptosis in Huh-7 hepatoma cells during arsenic trioxide exposure.
Biochem Biophys Res Commun
2023
651:92-97
PubMed ID: 36801614
DOI: 10.1016/j.bbrc.2023.02.020
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16391
Sakamoto T, Yu Z, Otani Y.
Dual-Color Fluorescence Switch-On Probe for Imaging G-Quadruplex and Double-Stranded DNA in Living Cells
Anal Chem
2022
Mar 2
PubMed ID: 35234461
DOI: 10.1021/acs.analchem.1c04804
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20771
Kobayashi-Sakamoto M, Maeda T, Kimura M, Yusa J, Ito H, Tani H, Kato Y, Hirose K.
Bovine lactoferrin increases the poly(I:C)-induced antiviral response in vitro.
Biochem Cell Biol
2022
PubMed ID: 35830725
DOI: 10.1139/bcb-2021-0342
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20774
Kubota Y, Fujioka Y, Patil A, Takagi Y, Matsubara D, Iijima M, Momose I, Naka R, Nakai K, Noda NN, Takekawa M.
Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.
Nat Commun
2022
13(1):4063
PubMed ID: 35831322
DOI: 10.1038/s41467-022-31690-w
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20891
Matsuya Y, Kai T, Parisi A, Yoshii Y, Sato T.
Application of a simple DNA damage model developed for electrons to proton irradiation.
Phys Med Biol
2022
PubMed ID: 36228611
DOI: 10.1088/1361-6560/ac9a20
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20954
Ohe S, Kubota Y, Yamaguchi K, Takagi Y, Nashimoto J, Kozuka-Hata H, Oyama M, Furukawa Y, Takekawa M.
ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II.
Nat Commun
2022
13(1):7476
PubMed ID: 36463234
DOI: 10.1038/s41467-022-35230-4
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20360
Kashio-Yokota Y, Sato S, Hirose T, Watanabe T, Endo A, Watanabe F, Endo M, Ohba K, Mori T, Takahashi K.
Elevated (Pro)renin Receptor Expression by Anti-Cancer Drugs, Carboplatin and Paclitaxel, in Cultured Cancer Cells: Possible Involvement of Apoptosis and Autophagy.
Tohoku J Exp Med
2021
255(2):91-104
PubMed ID: 34645770
DOI: 10.1620/tjem.255.91
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20756
Xie Y, Wang B, Du L, Wang Y, Xu C, Zhang H, Wen K, Liu Q, Katsube T.
ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines.
Cancer Biol Med
2021
PubMed ID: 34546667
DOI: 10.20892/j.issn.2095-3941.2020.0569
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13571
Melanny Ika Sulistyowaty et al
Synthesis, In Vitro Anticancer Activity and In Silico Study of some Benzylidene Hydrazide Derivatives
Key Engineering Materials
2020
840:277-283
DOI: 10.4028/www.scientific.net/KEM.840.277
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11381
Win NN, Kyaw MM, Prema, Ngwe H, Ito T, Asakawa Y, Okamoto Y, Tanaka M, Abe I, Morita H.
Dinorcassane Diterpenoid from Boesenbergia rotunda Rhizomes Collected in Lower Myanmar.
Chem. Biodivers.
2019
PubMed ID: 30645035
DOI: 10.1002/cbdv.201800657
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