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Cell No. : Cell Name
RCB3677 : A549  update : 2024/01/05
CommentHuman cell line derived from lung cancer. Epithelial-like cells. HLA-A 2603/3001. A culture submitted to the Cell Resource Center for Biomedical Research was found to be contaminated with mycoplasma. Progeny were cured by Treatments with BM Cycline and MC210. TKG0184 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Giard, D. J. et al.
Year of deposit 2011
Original cell TKG0184
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue lung
Disease name adenocarcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0184)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0023
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8-10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
deposit info
lot info
Reference information Reference 2
User's Publication 12

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4609  Lieber M, Smith B, Szakal A, Nelson-Rees W, Todaro G.  A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells.  Int J Cancer  1976  17(1):62-70  PubMed ID: 175022   DOI: 10.1002/ijc.2910170110
1026  Giard DJ, Aaronson SA, Todaro GJ, Arnstein P, Kersey JH, Dosik H, Parks WP.  In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.  J Natl Cancer Inst  1973  51(5):1417-23  PubMed ID: 4357758   DOI: 10.1093/jnci/51.5.1417

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User's Publication
17744  Kawasaki S, Ono H, Hirosawa M, Kuwabara T, Sumi S, Lee S, Woltjen K, Saito H.  Programmable mammalian translational modulators by CRISPR-associated proteins  Nat Commun  2023  14(1):2243  PubMed ID: 37076490   DOI: 10.1038/s41467-023-37540-7
19947  Moriizumi H, Kubota Y, Tsuchiya T, Naka R, Takekawa M.  Caspase 3-specific cleavage of MEK1 suppresses ERK signaling and sensitizes cells to stress-induced apoptosis  FEBS Open Bio  2023  13(4):684-700  PubMed ID: 36776127   DOI: 10.1002/2211-5463.13574
21118  Aki T, Kukita M, Takata M, Funakoshi T, Unuma K, Uemura K.  Induction of filamin-C and its involvement in the regulation of cellular senescence and apoptosis in Huh-7 hepatoma cells during arsenic trioxide exposure.  Biochem Biophys Res Commun  2023  651:92-97  PubMed ID: 36801614   DOI: 10.1016/j.bbrc.2023.02.020
16391  Sakamoto T, Yu Z, Otani Y.  Dual-Color Fluorescence Switch-On Probe for Imaging G-Quadruplex and Double-Stranded DNA in Living Cells  Anal Chem  2022  Mar 2  PubMed ID: 35234461   DOI: 10.1021/acs.analchem.1c04804
20771  Kobayashi-Sakamoto M, Maeda T, Kimura M, Yusa J, Ito H, Tani H, Kato Y, Hirose K.  Bovine lactoferrin increases the poly(I:C)-induced antiviral response in vitro.  Biochem Cell Biol  2022    PubMed ID: 35830725   DOI: 10.1139/bcb-2021-0342
20774  Kubota Y, Fujioka Y, Patil A, Takagi Y, Matsubara D, Iijima M, Momose I, Naka R, Nakai K, Noda NN, Takekawa M.  Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.  Nat Commun  2022  13(1):4063  PubMed ID: 35831322   DOI: 10.1038/s41467-022-31690-w
20891  Matsuya Y, Kai T, Parisi A, Yoshii Y, Sato T.  Application of a simple DNA damage model developed for electrons to proton irradiation.  Phys Med Biol  2022    PubMed ID: 36228611   DOI: 10.1088/1361-6560/ac9a20
20954  Ohe S, Kubota Y, Yamaguchi K, Takagi Y, Nashimoto J, Kozuka-Hata H, Oyama M, Furukawa Y, Takekawa M.  ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II.  Nat Commun  2022  13(1):7476  PubMed ID: 36463234   DOI: 10.1038/s41467-022-35230-4
20360  Kashio-Yokota Y, Sato S, Hirose T, Watanabe T, Endo A, Watanabe F, Endo M, Ohba K, Mori T, Takahashi K.  Elevated (Pro)renin Receptor Expression by Anti-Cancer Drugs, Carboplatin and Paclitaxel, in Cultured Cancer Cells: Possible Involvement of Apoptosis and Autophagy.  Tohoku J Exp Med  2021  255(2):91-104  PubMed ID: 34645770   DOI: 10.1620/tjem.255.91
20756  Xie Y, Wang B, Du L, Wang Y, Xu C, Zhang H, Wen K, Liu Q, Katsube T.  ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines.  Cancer Biol Med  2021    PubMed ID: 34546667   DOI: 10.20892/j.issn.2095-3941.2020.0569
13571  Melanny Ika Sulistyowaty et al  Synthesis, In Vitro Anticancer Activity and In Silico Study of some Benzylidene Hydrazide Derivatives  Key Engineering Materials  2020  840:277-283    DOI: 10.4028/
11381  Win NN, Kyaw MM, Prema, Ngwe H, Ito T, Asakawa Y, Okamoto Y, Tanaka M, Abe I, Morita H.  Dinorcassane Diterpenoid from Boesenbergia rotunda Rhizomes Collected in Lower Myanmar.  Chem. Biodivers.  2019    PubMed ID: 30645035   DOI: 10.1002/cbdv.201800657

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