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Cell No. : Cell Name
RCB3676 : 5637  update : 2022/01/20
CommentHuman cell line derived from bladder carcinoma. TKG0605 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Cannon G.
Year of deposit 2011
Original cell TKG0605
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 68 years
Tissue bladder
Disease name Bladder carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0605)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0126
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.02% EDTA-PBS
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 8
User's Publication 1


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Reference
4954  Quentmeier H, Zaborski M, Drexler HG.  The human bladder carcinoma cell line 5637 constitutively secretes functional cytokines.  Leuk Res  1997  21(4):343-50  PubMed ID: 9150352   DOI: 10.1016/s0145-2126(96)00132-4
4953  Kaashoek JG, Mout R, Falkenburg JH, Willemze R, Fibbe WE, Landegent JE.  Cytokine production by the bladder carcinoma cell line 5637: rapid analysis of mRNA expression levels using a cDNA-PCR procedure.  Lymphokine Cytokine Res  1991  10(3):231-5  PubMed ID: 1883917  
2036  Miyauchi J, Kelleher CA, Yang YC, Wong GG, Clark SC, Minden MD, Minkin S, McCulloch EA.  The effects of three recombinant growth factors, IL-3, GM-CSF, and G-CSF, on the blast cells of acute myeloblastic leukemia maintained in short-term suspension culture.  Blood  1987  70(3):657-63  PubMed ID: 3304459  
2037  Asano Y, Shibuya T, Okamura S, Morioka E, Taniguchi S, Niho Y.  Synergism of leukemic blast growth factors in medium conditioned by human bladder carcinoma cell line 5637.  Int J Cell Cloning  1987  5(6):504-10  PubMed ID: 3480913   DOI: 10.1002/stem.5530050608
2035  Welte K, Platzer E, Lu L, Gabrilove JL, Levi E, Mertelsmann R, Moore MA.  Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor.  Proc Natl Acad Sci U S A  1985  82(5):1526-30  PubMed ID: 3871951   DOI: 10.1073/pnas.82.5.1526
5464  Welte K, Platzer E, Gabrilove JL, Lu L, Levi E, Polivka A, Mertelsmann R, Moore MA.  Purification to apparent homogeneity and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor.  Haematol Blood Transfus  1985  29:398-401  PubMed ID: 3875526  
4956  Myers CD, Katz FE, Joshi G, Millar JL.  A cell line secreting stimulating factors for CFU-GEMM culture.  Blood  1984  64(1):152-5  PubMed ID: 6733267  
3355  Fogh J.  Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors.  Natl Cancer Inst Monogr  1978  49:5-9.  PubMed ID: 571047  

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User's Publication
14268  Yamashita T, Higashi M, Momose S, Morozumi M, Tamaru JI.  Nuclear expression of Y box binding-1 is important for resistance to chemotherapy including gemcitabine in TP53-mutated bladder cancer  Int J Oncol  2017  51(2):579-586  PubMed ID: 28714514   DOI: 10.3892/ijo.2017.4031



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