Cell No. : Cell Name RCB3673 : Raji  update : 2024/08/14
Comment			Human cell line derived from Burkitt lymphoma. EBNA (EBV—nuclear antigen) positive. TKG0371 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Fukuda, Hiroshi
		Originator	P. J. V. Pulvertaft
		Year of deposit	2011
		Original cell	TKG0371
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Age at sampling	11 years
		Tissue	left maxilla
		Disease name	Burkitt's Lymphoma
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0371）
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_0511
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 5 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Virus (HIV)		Undetected
		Virus (HTLV-1)		(-)
		Virus (EBV)		(+)
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	5
		User's Publication	2

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Reference

1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890   DOI: 10.1084/jem.143.6.1528

4739  Huang CC, Minowada J.  Differential effects of infection with herpes simplex virus on the chromosomes of human hematopoietic cell lines.  Cancer Res  1972  32(6):1218-25  PubMed ID: 4337885

2472      Trans N Y Acad Sci  1966  29:61

479  Epstein, M A, Achong, B G, Barr, Y M, Zajac B, Henle G, Henle W  Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji).  J Natl Cancer Inst  1966  37:547-59  PubMed ID: 4288580

972  Pulvertaft,R.J.V.  Cytology of Burkitt's tumor (African lymphoma).  Lancet  1964  1:238-240  PubMed ID: 14086209   DOI: 10.1016/s0140-6736(64)92345-1

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User's Publication

21441  Kanto N, Ohkawa Y, Kitano M, Maeda K, Shiida M, Ono T, Ota F, Kizuka Y, Kunimasa K, Nishino K, Mukai M, Seike M, Azuma A, Harada Y, Fukuda T, Gu J, Taniguchi N.  A highly specific antibody against the core fucose of the N-glycan in IgG identifies the pulmonary diseases and its regulation by CCL2.  J Biol Chem  2023    PubMed ID: 37865317   DOI: 10.1016/j.jbc.2023.105365

10518  Kiso K, Yoshifuji H, Oku T, Hikida M, Kitagori K, Hirayama Y, Nakajima T, Haga H, Tsuruyama T, Miyagawa-Hayashino A.  Transgelin-2 is upregulated on activated B-cells and expressed in hyperplastic follicles in lupus erythematosus patients.  PLoS ONE  2017  12:e0184738  PubMed ID: 28910360   DOI: 10.1371/journal.pone.0184738