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Cell No. : Cell Name
RCB3673 : Raji  update : 2020/09/24
CommentHuman cell line derived from Burkitt lymphoma. EBNA (EBV—nuclear antigen) positive. TKG0371 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator P. J. V. Pulvertaft
Year of deposit 2011
Original cell TKG0371
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 11 years
Tissue left maxilla
Case history Burkitt's Lymphoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0371)
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 5 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Images
deposit info
lot info
Reference information Reference 5
User's Publication 5


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Reference
1141  Ralph P, Moore, M A, Nilsson K  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines.  J Exp Med  1976  143:1528-33  PubMed ID: 1083890
4739  Huang CC, Minowada J.  Differential effects of infection with herpes simplex virus on the chromosomes of human hematopoietic cell lines.  Cancer Res  1972  32(6):1218-25  PubMed ID: 4337885
2472      Trans N Y Acad Sci  1966  29:61 
479  Epstein, M A, Achong, B G, Barr, Y M, Zajac B, Henle G, Henle W  Morphological and virological investigations on cultured Burkitt tumor lymphoblasts (strain Raji).  J Natl Cancer Inst  1966  37:547-59  PubMed ID: 4288580
972  Pulvertaft,R.J.V.  Cytology of Burkitt's tumor (African lymphoma).  Lancet  1964  1:238-240  PubMed ID: 14086209

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User's Publication
11509  Zhelev Z, Georgieva E, Lazarova D, Semkova S, Aoki I, Gulubova M, Higashi T, Bakalova R.  Redox Imaging to Distinguish Cells with Different Proliferative Indexes: Superoxide, Hydroperoxides, and Their Ratio as Potential Biomarkers.  Oxid Med Cell Longev  2019    PubMed ID: 31089411
10301  Goto H, Kariya R, Kudo E, Okuno Y, Ueda K, Katano H, Okada S.  Restoring PU.1 induces apoptosis and modulates viral transactivation via interferon-stimulated genes in primary effusion lymphoma.  Oncogene  2017  36:5252-5262  PubMed ID: 28481873
10518  Kiso K, Yoshifuji H, Oku T, Hikida M, Kitagori K, Hirayama Y, Nakajima T, Haga H, Tsuruyama T, Miyagawa-Hayashino A.  Transgelin-2 is upregulated on activated B-cells and expressed in hyperplastic follicles in lupus erythematosus patients.  PLoS ONE  2017  12:e0184738  PubMed ID: 28910360
7572  Yew WT, Kitson L, Hock AHS, Chiew GS, Mooi LY  2-Methoxy-1, 4-Naphthoquinone (MNQ) Suppresses Protein Kinase C βI, δ, and ζ Expression in Raji Cells.  J App Pharm Sci.  2015  5:001-005 
6060  Liew K, Yong PV, Lim YM, Navaratnam V, Ho AS.  2-Methoxy-1,4-Naphthoquinone (MNQ) suppresses the invasion and migration of a human metastatic breast cancer cell line (MDA-MB-231).  Toxicol In Vitro  2014  28(3):335-9  PubMed ID: 24291160



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