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Cell No. : Cell Name
RCB3600 : SDR-P-1D5  update : 2023/10/16
CommentRat prolactin secreting cell line derived from anterior pituitary lacking growth hormone.
Comment from the depositor
Terms and conditions1) As a rule, it makes joint researches and required written permission from the approver. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Endocrinology 1990:126:31-38.) designated by the DEPOSITOR is required. 3) Before apply for a patent, contact a person who establish a cell line.
Remarks
approver's address
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English
Address
Contact Us
cellbank.brc@riken.jp
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.ISHIKAWA Hiroshi (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ishikawa, Hiroshi
Originator Ishikawa, Hiroshi
Year of deposit 2011
Animal _rat < Mammals
Strain name SDR
Tissue Pituitary gland
Classification normal
Lifespan unknown
Morphology other
Cellosaurus(Expasy) CVCL_A6JE
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM/HamF12 + 15% FBS + 0.01mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
3640  Takeuchi T, Suzuki H, Sakurai S, Nogami H, Okuma S, Ishikawa H.  Molecular mechanism of growth hormone (GH) deficiency in the spontaneous dwarf rat: detection of abnormal splicing of GH messenger ribonucleic acid by the polymerase chain reaction.  Endocrinology  1990  126(1):31-8  PubMed ID: 2152867   DOI: 10.1210/endo-126-1-31

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