Cell No. : Cell Name
RCB3588 : hPSC-5 update : 2024/08/14 |
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Comment | Human cell line derived from pacreatic cancer. Pancreatic stellate cells. | |||
Comment from the depositor | ||||
Terms and conditions | 1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the approver. 2) Coauthorship must be given to the depositor when the results using the cells are published. | |||
Remarks | ||||
approver's address |
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Order Form | Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   | |||
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
Basic information | Depositor | Masamune, Atsushi | ||
Originator | Masamune, Atsushi | |||
Year of deposit | 2011 | |||
Animal | _human < Mammals | |||
Genus | Homo | |||
Species | sapiens | |||
Race | Japanese | |||
Gender | Female | |||
Tissue | pancreas | |||
Disease name | pancreas cancer | |||
Classification | normal | Lifespan | finite | |
Crisis PDL | 50 PDL | |||
Morphology | fibroblast-like | |||
Cellosaurus(Expasy) | CVCL_T769 | |||
Medium | Medium List | Culture type | Adherent cells | |
Culture medium | DMEM/HamF12 + 10% FBS | |||
Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 2.5 split |
SC frequency | Subculture : once/5 days | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
Animal PCR | OK | |||
STR(human) | OK | |||
Images | ||||
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Reference information | Reference | 1 | ||
User's Publication | 3 | |||