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Cell No. : Cell Name
RCB3588 : hPSC-5  update : 2022/05/18
CommentHuman cell line derived from pacreatic cancer. Pancreatic stellate cells.
Comment from the depositor
Terms and conditions1) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the approver. 2) Coauthorship must be given to the depositor when the results using the cells are published.
Remarks
approver's address
×
English
Address
Tohoku university hospital

1-1 Seiryo-cho, Aobaka-ku, Sendai Miyagi 980-8574 Japan
Dr.MASAMUNE Atsushi
Fax. +81-22-717-7177
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Masamune, Atsushi
Originator Masamune, Atsushi
Year of deposit 2011
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Tissue pancreas
Disease name pancreas cancer
Classification normal
Lifespan finite
Crisis PDL 50 PDL
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T769
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 2.5 split
SC frequency Subculture : once/5 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
16509  Oshima Y, Sano M, Kajiwara I, Ichimaru Y, Itaya T, Kuramochi T, Hayashi E, Kim J, Kitajima O, Masugi Y, Masamune A, Ijichi H, Ishii Y, Suzuki T.  Midazolam exhibits antitumour and anti-inflammatory effects in a mouse model of pancreatic ductal adenocarcinoma  Br J Anaesth  2022  128(4):679-690  PubMed ID: 35120712

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User's Publication
6912  Ishii N, Araki K, Yokobori T, Hagiwara K, Gantumur D, Yamanaka T, Handa T, Tsukagoshi M, Igarashi T, Watanabe A, Kubo N, Harimoto N, Masamune A, Umezawa K, Kuwano H, Shirabe K.  Conophylline suppresses pancreatic cancer desmoplasia and cancer-promoting cytokines produced by cancer-associated fibroblasts.  Cancer Sci  2019  110(1):334-344  PubMed ID: 30353606



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