Cell No. : Cell Name
RCB3580 : ASF-4-3L update : 2024/07/09 |
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Comment | Normal human skin fibroblast. RCB3579〜RCB3585 were derived from the same person. | |||
Comment from the depositor | ||||
Terms and conditions | A prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use. | |||
Remarks | ||||
approver's address |
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Order Form | Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   | |||
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
Basic information | Depositor | KAJI, Kazuhiko | ||
Originator | KAJI, Kazuhiko | |||
Year of deposit | 2011 | |||
Animal | _human < Mammals | |||
Strain name | Homo | |||
Genus | sapiens | |||
Race | Japanese | |||
Gender | Male | |||
Age at sampling | 56.9 years | |||
Tissue | skin | |||
Classification | normal | |||
Year of origin | 2000 | Lifespan | finite | |
Crisis PDL | 56.9 PDL | |||
Morphology | fibroblast-like | |||
Contact inhibition | Yes | |||
Tumorigenesis | No | |||
Cellosaurus(Expasy) | CVCL_8497 | |||
Medium | Medium List | Culture type | Adherent cells | Adherent cells |
Culture medium | MEM + 10% FBS | MEM + 10% FBS | ||
Antibiotics | Free | Free | Passage method | 0.25% Trypsin (7-10min, 37 ℃) | 0.25% Trypsin | Culture information | Passage cell No | 4-6x10 5 cells/60mm dish |
Passage ratio | 1 : 4 split | 1 : 4 split | ||
SC frequency | Subculture : once/week, Medium Renewal : once/3-4 days | Subculture : once/week, Medium Renewal : once/3-4 days | ||
Temperature | 37 ℃ | 37 ℃ | ||
CO2 concentration | 5 % | 5 % | ||
Freeze medium | MEM + 30% FBS + 10% DMSO | MEM + 30% FBS + 10% DMSO | ||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
Animal PCR | OK | |||
Chromosome mode | 46,XY | |||
Saturation density | 6-10x10 4 cells/cm 2 | |||
Doubling time | 20-24 hr | |||
STR(human) | OK | |||
HLA | A2, A24, Bw61 | |||
Reference information | Reference | 2 | ||
User's Publication | 1 | |||