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Cell No. : Cell Name
RCB3580 : ASF-4-3L  update : 2024/01/17
CommentNormal human skin fibroblast. RCB3579〜RCB3585 were derived from the same person.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Contact Us
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.KAJI Kazuhiko (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor KAJI, Kazuhiko
Originator KAJI, Kazuhiko
Year of deposit 2011
Animal _human < Mammals
Strain name Homo
Genus sapiens
Race Japanese
Gender Male
Age at sampling 56.9 years
Tissue skin
Classification normal
Year of origin 2000
Lifespan finite
Crisis PDL 56.9 PDL
Morphology fibroblast-like
Contact inhibition Yes
Tumorigenesis No
Cellosaurus(Expasy) CVCL_8497
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Culture medium MEM + 10% FBS MEM + 10% FBS
Antibiotics Free Free
Passage method 0.25% Trypsin (7-10min, 37 ℃) 0.25% Trypsin
Culture information Passage cell No 4-6x10 5 cells/60mm dish
Passage ratio 1 : 4 split 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : once/3-4 days Subculture : once/week, Medium Renewal : once/3-4 days
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium MEM + 30% FBS + 10% DMSO MEM + 30% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Chromosome mode 46,XY
Saturation density 6-10x10 4 cells/cm 2
Doubling time 20-24 hr
STR(human) OK
HLA A2, A24, Bw61
Reference information Reference 2
User's Publication 1

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20000  Itai E, Atsugi T, Inomata K, Yamashita M, Kaji K, Nanba D, Naru E.  Single-cell analysis of human dermal fibroblasts isolated from a single male donor over 35 years  Exp Dermatol  2023  32(11):1982-1995  PubMed ID: 37727050   DOI: 10.1111/exd.14929
7443  Kaji K, Ohta T, Horie N, Naru E, Hasegawa M, Kanda N.  Donor age reflects the replicative lifespan of human fibroblasts in culture.  Hum Cell  2009  22(2):38-42  PubMed ID: 19385098   DOI: 10.1111/j.1749-0774.2009.00066.x

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User's Publication
19999  Segawa K, Kikuchi A, Noji T, Sugiura Y, Hiraga K, Suzuki C, Haginoya K, Kobayashi Y, Matsunaga M, Ochiai Y, Yamada K, Nishimura T, Iwasawa S, Shoji W, Sugihara F, Nishino K, Kosako H, Ikawa M, Uchiyama Y, Suematsu M, Ishikita H, Kure S, Nagata S.  A sublethal ATP11A mutation associated with neurological deterioration causes aberrant phosphatidylcholine flipping in plasma membranes  J Clin Invest  2021  131(18):e148005  PubMed ID: 34403372   DOI: 10.1172/JCI148005

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