Cell No. : Cell Name
RCB3547 : Sez627(+)
update : 2024/11/11
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| Comment | BSL2. We don't know whether virus particles are produced or not. Therefore, the cells must be handled under BSL2. |
| Comment from the depositor | Sezary syndrome like patient derived T cell line (IL-2 dependent). |
| Terms and conditions | 1) The material will be used for teaching or not-for profit research purpose only. To use the material for commercial purpose, he/she should refer to the establisher of the material. 2) The material will not be further distributed to others without the provider’s written consent. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Cell line to be handled at BSL 2(C-0070.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
MAEDA, Michiyuki
|
| Originator |
MAEDA, Michiyuki
|
| Year of deposit |
2011
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Tissue |
peripheral blood
|
| Disease name |
Sezary syndrome
|
| Classification |
cancer
|
| Year of origin |
1983
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Product |
HTLV-1 pro-virus DNA(+)
|
| Surface antigen (depositor) |
T cell marker (+), CD25 (+), HLA-DR (+)
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_5364
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Suspension cells
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
10%FCS, RPMI1640, 7.5-15ng/ml human rIL-2
|
RPMI1640 + 10% FBS + 15ng/ml human rIL-2
|
| Antibiotics |
100μg/ml kanamycin
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage cell No |
1-2 x 10 5 cells/ml
|
|
| Passage ratio |
1 : 5-10 split
|
1 : 2 split
|
| SC frequency |
Subculture : 2 times/week
|
Subculture : 1-2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
DNA (+)
|
| Virus (EBV) |
|
(-)
|
| Saturation density |
5-10 x 10 5 cells/ml
|
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
2761
K Nosaka, M Maeda, S Tamiya, T Sakai, H Mitsuya, M Matsuoka
Increasing methylation of the CDKN2A gene is associated with the progression of adult T-cell leukemia
Cancer Res
2000
60(4):1043-8
PubMed ID: 10706122
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2510
Maeda M, Noma T, Hama K, Honjo T.
Application of a human T cell line derived from a Sézary syndrome patient for human interleukin 4 assay
Immunol Lett
1988
18(4):247-53
PubMed ID: 3263317
DOI: 10.1016/0165-2478(88)90170-8
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3090
S Namiuchi, S Kumagai, H Sano, J Yodoi, T Uchiyama, K Ikai, H Imura, M Maeda
A human T cell line established from a patient with Sézary syndrome. Application for assay of human interleukin 2 (IL-2)
J Immunol Methods
1986
94(1-2):215-24
PubMed ID: 3023492
DOI: 10.1016/0022-1759(86)90236-x
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