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Cell No. : Cell Name
RCB3542 : ATL-72/2(-)  update : 2024/01/17
Comment from the depositorATL patient derived non-leukemic cell line (IL-2 independent). ATL-72/2(+), ATL-72/2(-), ATL-72b(+), ATL-72b(-), ATL-72T(+) cell lines were established from the same patient's blood.
Terms and conditions1) The material will be used for teaching or not-for profit research purpose only. To use the material for commercial purpose, he/she should refer to the establisher of the material. 2) The material will not be further distributed to others without the provider’s written consent.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor MAEDA, Michiyuki
Originator MAEDA, Michiyuki
Year of deposit 2011
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue peripheral blood
Disease name ATL
Classification cancer
Year of origin 1998
Lifespan infinite
Morphology lymphocyte-like
Product HTLV-1 pro-virus DNA(+)
Surface antigen (depositor) T cell marker (+), CD25 (+), HLA-DR (+)
Cellosaurus(Expasy) CVCL_A6TE
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Culture medium 10%FCS, RPMI1640 RPMI1640 + 10% FBS
Culture method
Antibiotics 100μg/ml kanamycin Free
Passage method dilution
Culture information Passage cell No 1-2 x 10 5 cells/ml
Passage ratio 1 : 5-10 split 1 : 5-10 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) DNA (+)
Virus (EBV) (-)
Saturation density 5-10 x 10 5 cells/ml
STR(human) OK
deposit info
lot info
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
9 8,11 12 10 14,16 6,9 X 11 10,12
Reference information Reference 1
User's Publication 0

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3175  Michiyuki Maeda, Junko Tanabe-Shibuya, Paola Miyazato, Hiroshi Masutani, Jun-Ichirou Yasunaga, Kazumasa Usami, Akira Shimizu, Masao Matsuoka  IL-2/IL-2 Receptor Pathway Plays a Crucial Role in the Growth and Malignant Transformation of HTLV-1-Infected T Cells to Develop Adult T-Cell Leukemia  Front Microbiol  2020  11:356  PubMed ID: 32210945   DOI: 10.3389/fmicb.2020.00356

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