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Cell No. : Cell Name
RCB3539 : ED-50823(+)  update : 2022/11/07
CommentATL patient derived non-leukemic cell line (IL-2 dependent).
Comment from the depositorATL patient derived non-leukemic cell line (IL-2 dependent). ED-40515, ED-40810, ED-41214, ED-50823, ED-70423C cell lines were established from the same patient's blood.
Terms and conditions1) The material will be used for teaching or not-for profit research purpose only. To use the material for commercial purpose, he/she should refer to the establisher of the material. 2) The material will not be further distributed to others without the provider’s written consent.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor MAEDA, Michiyuki
Originator MAEDA, Michiyuki
Year of deposit 2011
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue peripheral blood
Disease name ATL
Classification cancer
Year of origin 1985
Lifespan infinite
Morphology lymphocyte-like
Product HTLV-1 pro-virus DNA(+)
Surface antigen (depositor) T cell marker (+), CD25 (+), HLA-DR (+)
BSL BSL2
Cellosaurus(Expasy) CVCL_A6SK
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives 10%FCS, RPMI1640, 7.5-15mg/ml human rIL-2 RPMI1640 + 10% FBS + 15ng/ml human IL-2
Antibiotics 100μg/ml kanamycin Free
Passage method dilution
Culture information Passage cell No 1-2 x 10^(5) cells/ml
Passage ratio 1 : 5-10 split 1 : 3-5 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) DNA (+)
Saturation density 5-10 x 10^(5) cells/ml
STR(human) OK
Reference information Reference 1
User's Publication 0


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Reference
3175  Michiyuki Maeda, Junko Tanabe-Shibuya, Paola Miyazato, Hiroshi Masutani, Jun-Ichirou Yasunaga, Kazumasa Usami, Akira Shimizu, Masao Matsuoka  IL-2/IL-2 Receptor Pathway Plays a Crucial Role in the Growth and Malignant Transformation of HTLV-1-Infected T Cells to Develop Adult T-Cell Leukemia  Front Microbiol  2020  11:356  PubMed ID: 32210945

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