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Cell No. : Cell Name
RCB3537 : ATL-55T(+)  update : 2024/01/17
Comment from the depositorATL patient derived leukemic cell line (IL-2 dependent). ATL-55T(+), ATL-55T(-) cell lines were established from the same patient's blood.
Terms and conditions1) The material will be used for teaching or not-for profit research purpose only. To use the material for commercial purpose, he/she should refer to the establisher of the material. 2) The material will not be further distributed to others without the provider’s written consent.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor MAEDA, Michiyuki
Originator MAEDA, Michiyuki
Year of deposit 2011
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue peripheral blood
Disease name ATL
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Product HTLV-1 pro-virus DNA(+)
Surface antigen (depositor) T cell marker (+), CD25 (+), HLA-DR (+)
Cellosaurus(Expasy) CVCL_VQ17
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Culture medium 10%FCS, RPMI1640, 7.5-15mg/ml human rIL-2 RPMI1640 + 10% FBS + 15ng/ml human rIL-2
Culture method
Antibiotics 100μg/ml kanamycin Free
Passage method dilution
Culture information Passage cell No 1-2 x 10 5 cells/ml
Passage ratio 1 : 5-10 split 1 : 8-12 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) DNA (+)
Virus (EBV) (-)
Saturation density 5-10 x 10 5 cells/ml
STR(human) OK
deposit info
lot info
D5S818 D13S317 D7S820 D16S539 vWA TH01 Amelogenin TPOX CSF1PO
10,11 10 10,11 9 16,18 7,8 X 8,12 11,12
Reference information Reference 4
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17268  Chiba M, Shimono J, Ishio T, Takei N, Kasahara K, Ogasawara R, Ara T, Goto H, Izumiyama K, Otsuguro S, Perera LP, Hasegawa H, Maeda M, Hashino S, Maenaka K, Teshima T, Waldmann TA, Yang Y, Nakagawa M.  Genome-wide CRISPR screens identify CD48 defining susceptibility to NK cytotoxicity in peripheral T-cell lymphomas  Blood  2022  2022 Aug 3;blood.2022015646  PubMed ID: 35921533   DOI: 10.1182/blood.2022015646
3175  Michiyuki Maeda, Junko Tanabe-Shibuya, Paola Miyazato, Hiroshi Masutani, Jun-Ichirou Yasunaga, Kazumasa Usami, Akira Shimizu, Masao Matsuoka  IL-2/IL-2 Receptor Pathway Plays a Crucial Role in the Growth and Malignant Transformation of HTLV-1-Infected T Cells to Develop Adult T-Cell Leukemia  Front Microbiol  2020  11:356  PubMed ID: 32210945   DOI: 10.3389/fmicb.2020.00356
2858  Satoshi Takeda, Michiyuki Maeda, Shigeru Morikawa, Yuko Taniguchi, Jun-Ichirou Yasunaga, Kisato Nosaka, Yuetsu Tanaka, Masao Matsuoka  Genetic and epigenetic inactivation of tax gene in adult T-cell leukemia cells  Int J Cancer  2004  109(4):559-67  PubMed ID: 14991578   DOI: 10.1002/ijc.20007
2761  K Nosaka, M Maeda, S Tamiya, T Sakai, H Mitsuya, M Matsuoka  Increasing methylation of the CDKN2A gene is associated with the progression of adult T-cell leukemia  Cancer Res  2000  60(4):1043-8  PubMed ID: 10706122  

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