Cell No. : Cell Name
RCB3534 : ED-40515(-)
update : 2024/11/11
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| Comment | BSL2. We don't know whether virus particles are produced or not. Therefore, the cells must be handled under BSL2. |
| Comment from the depositor | ATL patient derived leukemic cell line (IL-2 independent). ED-40515, ED-40810, ED-41214, ED-50823, ED-70423C cell lines were established from the same patient's blood. |
| Terms and conditions | 1) The material will be used for teaching or not-for profit research purpose only. To use the material for commercial purpose, he/she should refer to the establisher of the material. 2) The material will not be further distributed to others without the provider’s written consent. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Cell line to be handled at BSL 2(C-0070.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
MAEDA, Michiyuki
|
| Originator |
MAEDA, Michiyuki
|
| Year of deposit |
2011
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Tissue |
peripheral blood
|
| Disease name |
ATL
|
| Classification |
cancer
|
| Year of origin |
1984
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Product |
HTLV-1 pro-virus DNA(+)
|
| Surface antigen (depositor) |
T cell marker (+), CD25 (+), HLA-DR (+)
|
| Tumorigenesis |
Yes
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_JF70
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Suspension cells
|
Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
10%FCS, RPMI1640
|
RPMI1640 + 10% FBS
|
| Antibiotics |
100μg/ml kanamycin
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage cell No |
1-2 x 10 5 cells/ml
|
|
| Passage ratio |
1 : 5-10 split
|
1 : 10-20 split
|
| SC frequency |
Subculture : 2 times/week
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER
|
CELLBANKER 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Virus (HIV) |
|
Undetected
|
| Virus (HTLV-1) |
|
DNA (+)
|
| Virus (EBV) |
|
(-)
|
| Saturation density |
5-10 x 10 5 cells/ml
|
|
| STR(human) |
|
OK
|
|
deposit info
|
|
|
lot info
|
| D5S818 |
D13S317 |
D7S820 |
D16S539 |
vWA |
TH01 |
Amelogenin |
TPOX |
CSF1PO |
|
11,13
|
8,12
|
8,11
|
12
|
14,18
|
9
|
X,Y
|
8,11
|
10
|
|
| Reference information |
Reference |
10
|
| User's Publication |
0
|
| Reference |
|
17268
Chiba M, Shimono J, Ishio T, Takei N, Kasahara K, Ogasawara R, Ara T, Goto H, Izumiyama K, Otsuguro S, Perera LP, Hasegawa H, Maeda M, Hashino S, Maenaka K, Teshima T, Waldmann TA, Yang Y, Nakagawa M.
Genome-wide CRISPR screens identify CD48 defining susceptibility to NK cytotoxicity in peripheral T-cell lymphomas
Blood
2022
2022 Aug 3;blood.2022015646
PubMed ID: 35921533
DOI: 10.1182/blood.2022015646
|
|
3175
Michiyuki Maeda, Junko Tanabe-Shibuya, Paola Miyazato, Hiroshi Masutani, Jun-Ichirou Yasunaga, Kazumasa Usami, Akira Shimizu, Masao Matsuoka
IL-2/IL-2 Receptor Pathway Plays a Crucial Role in the Growth and Malignant Transformation of HTLV-1-Infected T Cells to Develop Adult T-Cell Leukemia
Front Microbiol
2020
11:356
PubMed ID: 32210945
DOI: 10.3389/fmicb.2020.00356
|
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2505
Ahsan MK, Masutani H, Yamaguchi Y, Kim YC, Nosaka K, Matsuoka M, Nishinaka Y, Maeda M, Yodoi J.
Loss of interleukin-2-dependency in HTLV-I-infected T cells on gene silencing of thioredoxin-binding protein-2
Oncogene
2006
25(15):2181-91
PubMed ID: 16314839
DOI: 10.1038/sj.onc.1209256
|
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2502
Dewan MZ, Terashima K, Taruishi M, Hasegawa H, Ito M, Tanaka Y, Mori N, Sata T, Koyanagi Y, Maeda M, Kubuki Y, Okayama A, Fujii M, Yamamoto N.
Rapid tumor formation of human T-cell leukemia virus type 1-infected cell lines in novel NOD-SCID/gammac(null) mice: suppression by an inhibitor against NF-kappaB
J Virol
2003
77(9):5286-94
PubMed ID: 12692230
DOI: 10.1128/jvi.77.9.5286-5294.2003
|
|
2507
Kawamata S, Sakaida H, Hori T, Maeda M, Uchiyama T.
The upregulation of p27Kip1 by rapamycin results in G1 arrest in exponentially growing T-cell lines
Blood
1998
91(2):561-9
PubMed ID: 9427710
|
|
2509
Hiraiwa N, Dohi T, Kawakami-Kimura N, Yumen M, Ohmori K, Maeda M, Kannagi R.
Suppression of sialyl Lewis X expression and E-selectin-mediated cell adhesion in cultured human lymphoid cells by transfection of antisense cDNA of an alpha1-->3 fucosyltransferase (Fuc-T VII)
J Biol Chem
1996
271(49):31556-61
PubMed ID: 8940172
DOI: 10.1074/jbc.271.49.31556
|
|
2511
Ishii N, Asao H, Kimura Y, Takeshita T, Nakamura M, Tsuchiya S, Konno T, Maeda M, Uchiyama T, Sugamura K.
Impairment of ligand binding and growth signaling of mutant IL-2 receptor gamma-chains in patients with X-linked severe combined immunodeficiency
J Immunol
1994
153(3):1310-7
PubMed ID: 8027558
|
|
2506
Ohmori K, Takada A, Ohwaki I, Takahashi N, Furukawa Y, Maeda M, Kiso M, Hasegawa A, Kannagi M, Kannagi R.
A distinct type of sialyl Lewis X antigen defined by a novel monoclonal antibody is selectively expressed on helper memory T cells
Blood
1993
82(9):2797-805
PubMed ID: 7693048
|
|
2510
Maeda M, Noma T, Hama K, Honjo T.
Application of a human T cell line derived from a Sézary syndrome patient for human interleukin 4 assay
Immunol Lett
1988
18(4):247-53
PubMed ID: 3263317
DOI: 10.1016/0165-2478(88)90170-8
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2501
Maeda M, Shimizu A, Ikuta K, Okamoto H, Kashihara M, Uchiyama T, Honjo T, Yodoi J.
Origin of human T-lymphotrophic virus I-positive T cell lines in adult T cell leukemia. Analysis of T cell receptor gene rearrangement
J Exp Med
1985
162(6):2169-74.
PubMed ID: 2866223
DOI: 10.1084/jem.162.6.2169
|
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