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Cell No. : Cell Name
RCB3533 : ED-40515(+)  update : 2022/11/07
CommentATL patient derived leukemic cell line (IL-2 dependent).
Comment from the depositorATL patient derived leukemic cell line (IL-2 dependent). ED-40515, ED-40810, ED-41214, ED-50823, ED-70423C cell lines were established from the same patient's blood.
Terms and conditions1) The material will be used for teaching or not-for profit research purpose only. To use the material for commercial purpose, he/she should refer to the establisher of the material. 2) The material will not be further distributed to others without the provider’s written consent.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor MAEDA, Michiyuki
Originator MAEDA, Michiyuki
Year of deposit 2011
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue peripheral blood
Disease name ATL
Classification cancer
Year of origin 1984
Lifespan infinite
Morphology lymphocyte-like
Product HTLV-1 pro-virus DNA(+)
Surface antigen (depositor) T cell marker (+), CD25 (+), HLA-DR (+)
BSL BSL2
Cellosaurus(Expasy) CVCL_JF68
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives 10%FCS, RPMI1640, 7.5-15ng/ml human rIL-2 RPMI1640 + 10% FBS + 15ng/ml human IL-2
Antibiotics 100μg/ml kanamycin Free
Passage method dilution
Culture information Passage cell No 1-2 x 10^(5) cells/ml
Passage ratio 1 : 5-10 split 1 : 8 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER CELLBANKER 1
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) Undetected
Virus (HTLV-1) DNA (+)
Saturation density 5-10 x 10^(5) cells/ml
STR(human) OK
Reference information Reference 8
User's Publication 0


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Reference
17268  Chiba M, Shimono J, Ishio T, Takei N, Kasahara K, Ogasawara R, Ara T, Goto H, Izumiyama K, Otsuguro S, Perera LP, Hasegawa H, Maeda M, Hashino S, Maenaka K, Teshima T, Waldmann TA, Yang Y, Nakagawa M.  Genome-wide CRISPR screens identify CD48 defining susceptibility to NK cytotoxicity in peripheral T-cell lymphomas  Blood  2022  2022 Aug 3;blood.2022015646  PubMed ID: 35921533
3175  Michiyuki Maeda, Junko Tanabe-Shibuya, Paola Miyazato, Hiroshi Masutani, Jun-Ichirou Yasunaga, Kazumasa Usami, Akira Shimizu, Masao Matsuoka  IL-2/IL-2 Receptor Pathway Plays a Crucial Role in the Growth and Malignant Transformation of HTLV-1-Infected T Cells to Develop Adult T-Cell Leukemia  Front Microbiol  2020  11:356  PubMed ID: 32210945
2508  Matsuoka S, Ishii Y, Nakao A, Abe M, Ohtsuji N, Momose S, Jin H, Arase H, Sugimoto K, Nakauchi Y, Masutani H, Maeda M, Yagita H, Komatsu N, Hino O.  Establishment of a Therapeutic Anti-Pan HLA-Class II Monoclonal Antibody That Directly Induces Lymphoma Cell Death via Large Pore Formation  PLoS One  2016  11(3):e0150496.  PubMed ID: 27028595
2505  Ahsan MK, Masutani H, Yamaguchi Y, Kim YC, Nosaka K, Matsuoka M, Nishinaka Y, Maeda M, Yodoi J.  Loss of interleukin-2-dependency in HTLV-I-infected T cells on gene silencing of thioredoxin-binding protein-2  Oncogene  2006  25(15):2181-91  PubMed ID: 16314839
2503  Ueda M, Imada K, Imura A, Koga H, Hishizawa M, Uchiyama T.  Expression of functional interleukin-21 receptor on adult T-cell leukaemia cells  Br J Haematol  2005  128(2):169-76  PubMed ID: 15638850
2509  Hiraiwa N, Dohi T, Kawakami-Kimura N, Yumen M, Ohmori K, Maeda M, Kannagi R.  Suppression of sialyl Lewis X expression and E-selectin-mediated cell adhesion in cultured human lymphoid cells by transfection of antisense cDNA of an alpha1-->3 fucosyltransferase (Fuc-T VII)  J Biol Chem  1996  271(49):31556-61  PubMed ID: 8940172
2506  Ohmori K, Takada A, Ohwaki I, Takahashi N, Furukawa Y, Maeda M, Kiso M, Hasegawa A, Kannagi M, Kannagi R.  A distinct type of sialyl Lewis X antigen defined by a novel monoclonal antibody is selectively expressed on helper memory T cells  Blood  1993  82(9):2797-805  PubMed ID: 7693048
2501  Maeda M, Shimizu A, Ikuta K, Okamoto H, Kashihara M, Uchiyama T, Honjo T, Yodoi J.  Origin of human T-lymphotrophic virus I-positive T cell lines in adult T cell leukemia. Analysis of T cell receptor gene rearrangement  J Exp Med  1985  162(6):2169-74.  PubMed ID: 2866223

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