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Cell No. : Cell Name
RCB3532 : A129L  update : 2023/10/16
CommentHuman cell line derived from lung adenocarcinoma cells.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Chest 2002;122:282-288.) designated by the DEPOSITOR is required.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hanagiri, Takeshi
Originator Takenoyama, Mitsuhiro
Year of deposit 2010
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 64 years
Tissue lung
Disease name adenocarcinoma
Classification cancer
Year of origin 1996
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_U094
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10mM HEPES + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4-6 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
deposit info
lot info
Reference information Reference 11
User's Publication 1

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8991  Manabu Yasuda, Takeshi Hanagiri, Yoshiki Shigematsu, Takamitsu Onitsuka, Kouji Kuroda, Tetsurou Baba, Makiko Mizukami, Yoshinobu Ichiki, Hidetaka Uramoto, Mitsuhiro Takenoyama, Kosei Yasumoto  Identification of a Tumour Associated Antigen in Lung Cancer Patients With Asbestos Exposure  Anticancer Res  2010  30 (7), 2631-9  PubMed ID: 20682992  
8990  Kosei Yasumoto, Takeshi Hanagiri, Mitsuhiro Takenoyama  Lung Cancer-Associated Tumor Antigens and the Present Status of Immunotherapy Against Non-Small-Cell Lung Cancer  Gen Thorac Cardiovasc Surg  2009  57 (9), 449-57  PubMed ID: 19756930   DOI: 10.1007/s11748-008-0433-6
3628  Baba T, Hanagiri T, Ichiki Y, Kuroda K, Shigematsu Y, Mizukami M, Sugaya M, Takenoyama M, Sugio K, Yasumoto K.  Lack and restoration of sensitivity of lung cancer cells to cellular attack with special reference to expression of human leukocyte antigen class I and/or major histocompatibility complex class I chain related molecules A/B.  Cancer Sci  2007  98(11):1795-802  PubMed ID: 17725806   DOI: 10.1111/j.1349-7006.2007.00586.x
3637  Sugaya M, Takenoyama M, Shigematsu Y, Baba T, Fukuyama T, Nagata Y, Mizukami M, So T, Ichiki Y, Yasuda M, So T, Hanagiri T, Sugio K, Yasumoto K.  Identification of HLA-A24 restricted shared antigen recognized by autologous cytotoxic T lymphocytes from a patient with large cell carcinoma of the lung.  Int J Cancer  2007  120(5):1055-62  PubMed ID: 17131342   DOI: 10.1002/ijc.22396
8995  Takashi Fukuyama, Yoshinobu Ichiki, Sousuke Yamada, Yoshiki Shigematsu, Tetsuro Baba, Yoshika Nagata, Makiko Mizukami, Masakazu Sugaya, Mitsuhiro Takenoyama, Takeshi Hanagiri, Kenji Sugio, Kosei Yasumoto  Cytokine Production of Lung Cancer Cell Lines: Correlation Between Their Production and the Inflammatory/Immunological Responses Both in Vivo and in Vitro  Cancer Sci  2007  98 (7), 1048-54  PubMed ID: 17511773   DOI: 10.1111/j.1349-7006.2007.00507.x
9004  Makiko Mizukami, Takeshi Hanagiri, Manabu Yasuda, Koji Kuroda, Yoshiki Shigematsu, Tetsuro Baba, Takashi Fukuyama, Yoshika Nagata, Tetsuya So, Yoshinobu Ichiki, Masakazu Sugaya, Tomoko So, Mitsuhiro Takenoyama, Kenji Sugio, Kosei Yasumoto  Antitumor Effect of Antibody Against a SEREX-defined Antigen (UOEH-LC-1) on Lung Cancer Xenotransplanted Into Severe Combined Immunodeficiency Mice  Cancer Res  2007  67 (17), 8351-7  PubMed ID: 17804751   DOI: 10.1158/0008-5472.CAN-06-3889
8988  Takashi Fukuyama, Takeshi Hanagiri, Mitsuhiro Takenoyama, Yoshinobu Ichiki, Makiko Mizukami, Tetsuya So, Masakazu Sugaya, Tomoko So, Kenji Sugio, Kosei Yasumoto  Identification of a New Cancer/Germline Gene, KK-LC-1, Encoding an Antigen Recognized by Autologous CTL Induced on Human Lung Adenocarcinoma  Cancer Res  2006  66 (9), 4922-8  PubMed ID: 16651449   DOI: 10.1158/0008-5472.CAN-05-3840
7413  Mizukami M, Hanagiri T, Baba T, Fukuyama T, Nagata Y, So T, Ichiki Y, Sugaya M, Yasuda M, Takenoyama M, Sugio K, Yasumoto K.  Identification of tumor associated antigens recognized by IgG from tumor-infiltrating B cells of lung cancer: correlation between Ab titer of the patient's sera and the clinical course.  Cancer Sci  2005  96(12):882-8  PubMed ID: 16367908   DOI: 10.1111/j.1349-7006.2005.00119.x
8998  Yoshinobu Ichiki, Takeshi Hanagiri, Mitsuhiro Takenoyama, Tetsuro Baba, Takashi Fukuyama, Yoshika Nagata, Makiko Mizukami, Tetsuya So, Masakazu Sugaya, Manabu Yasuda, Tomoko So, Kenji Sugio, Kosei Yasumoto  Tumor Specific Expression of survivin-2B in Lung Cancer as a Novel Target of Immunotherapy  Lung Cancer  2005  48 (2), 281-9  PubMed ID: 15829330   DOI: 10.1016/j.lungcan.2004.10.017
3638  Sugaya M, Takenoyama M, Osaki T, Yasuda M, Nagashima A, Sugio K, Yasumoto K.  Establishment of 15 cancer cell lines from patients with lung cancer and the potential tools for immunotherapy.  Chest  2002  122(1):282-8  PubMed ID: 12114371   DOI: 10.1378/chest.122.1.282
8994  R Eifuku, M Takenoyama, I Yoshino, S Imahayashi, T So, M Yasuda, M Sugaya, K Yasumoto  Analysis of MAGE-3 Derived Synthetic Peptide as a Human Lung Cancer Antigen Recognized by Cytotoxic T Lymphocytes  Int J Clin Oncol  2001  6 (1), 34-9  PubMed ID: 11706525   DOI: 10.1007/pl00012077

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User's Publication
9006  Lilong Xia, Xinhai Zhu 1, Lei Zhang 1, Yanhui Xu 1, Guoping Chen 1, Jing Luo 1  EZH2 Enhances Expression of CCL5 to Promote Recruitment of Macrophages and Invasion in Lung Cancer  Biotechnol Appl Biochem  2020  67(6):1011-1019  PubMed ID: 31855281   DOI: 10.1002/bab.1875

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