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細胞材料の再培養方法 ( HPS以外・ HPS )
細胞番号 : 細胞名
RCB3529 : G603L  update : 2023/10/16
細胞特性(Comment:英)Human cell line derived from lung large cell carcinoma cells.
細胞特性(日)ヒト肺大細胞癌由来細胞株。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Cancer Sci, 2005;96:882-8 ) designated by the DEPOSITOR is required.
使用条件(日)利用者は、研究成果の公表にあたって寄託者の指定する文献 (Cancer Sci, 2005;96:882-8 ) を引用すること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 HANAGIRI, Takeshi
樹立者 TAKENOYAMA, Mitsuhiro
寄託日 2010
動物種 _human < Mammals
属名 Homo
種名 sapiens
人種 Japanese
性別 Male
齢 75 years
採取組織 lung
病名 large cell carcinoma
転移 Unknown
細胞分類 cancer
初代培養日 2002
細胞寿命 infinite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_U091
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 RPMI1640 + 10mM HEPES + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
動物種PCR OK
個体識別検査 OK
文献情報 Reference(英) 12件
Reference(日) 0件
利用者成果(英) 0件
利用者成果(日) 0件

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Reference(英)
8987  Tetsuro Baba, Takeshi Hanagiri, Mitsuhiro Takenoyama, Hironobu Shiota, Koji Kuroda, Yoshiki Shigematsu, Yoshinobu Ichiki, Hidetaka Uramoto, Tomoko So, Kosei Yasumoto  Identification of a Lung Cancer Antigen Evading CTL Attack Due to Loss of Human Leukocyte Antigen (HLA) Class I Expression  Cancer Sci  2010  101 (10), 2115-20  PubMed ID: 20649604   DOI: 10.1111/j.1349-7006.2010.01659.x
8991  Manabu Yasuda, Takeshi Hanagiri, Yoshiki Shigematsu, Takamitsu Onitsuka, Kouji Kuroda, Tetsurou Baba, Makiko Mizukami, Yoshinobu Ichiki, Hidetaka Uramoto, Mitsuhiro Takenoyama, Kosei Yasumoto  Identification of a Tumour Associated Antigen in Lung Cancer Patients With Asbestos Exposure  Anticancer Res  2010  30 (7), 2631-9  PubMed ID: 20682992  
8992  Koji Kuroda, Mitsuhiro Takenoyama, Tetsuro Baba, Yoshiki Shigematsu, Hironobu Shiota, Yoshinobu Ichiki, Manabu Yasuda, Hidetaka Uramoto, Takeshi Hanagiri, Kosei Yasumoto  Identification of Ribosomal Protein L19 as a Novel Tumor Antigen Recognized by Autologous Cytotoxic T Lymphocytes in Lung Adenocarcinoma  Cancer Sci  2010  101 (1), 46-53  PubMed ID: 19799608   DOI: 10.1111/j.1349-7006.2009.01351.x
8999  Hidetaka Uramoto, Hidehiko Shimokawa, Yoshika Nagata, Kenji Ono, Takeshi Hanagiri  EGFR-activating Mutations Are Not Present in Breast Tumors of Japanese Patients  Anticancer Res  2010  30 (10), 4219-22  PubMed ID: 21036744  
8990  Kosei Yasumoto, Takeshi Hanagiri, Mitsuhiro Takenoyama  Lung Cancer-Associated Tumor Antigens and the Present Status of Immunotherapy Against Non-Small-Cell Lung Cancer  Gen Thorac Cardiovasc Surg  2009  57 (9), 449-57  PubMed ID: 19756930   DOI: 10.1007/s11748-008-0433-6
3628  Baba T, Hanagiri T, Ichiki Y, Kuroda K, Shigematsu Y, Mizukami M, Sugaya M, Takenoyama M, Sugio K, Yasumoto K.  Lack and restoration of sensitivity of lung cancer cells to cellular attack with special reference to expression of human leukocyte antigen class I and/or major histocompatibility complex class I chain related molecules A/B.  Cancer Sci  2007  98(11):1795-802  PubMed ID: 17725806   DOI: 10.1111/j.1349-7006.2007.00586.x
3637  Sugaya M, Takenoyama M, Shigematsu Y, Baba T, Fukuyama T, Nagata Y, Mizukami M, So T, Ichiki Y, Yasuda M, So T, Hanagiri T, Sugio K, Yasumoto K.  Identification of HLA-A24 restricted shared antigen recognized by autologous cytotoxic T lymphocytes from a patient with large cell carcinoma of the lung.  Int J Cancer  2007  120(5):1055-62  PubMed ID: 17131342   DOI: 10.1002/ijc.22396
8995  Takashi Fukuyama, Yoshinobu Ichiki, Sousuke Yamada, Yoshiki Shigematsu, Tetsuro Baba, Yoshika Nagata, Makiko Mizukami, Masakazu Sugaya, Mitsuhiro Takenoyama, Takeshi Hanagiri, Kenji Sugio, Kosei Yasumoto  Cytokine Production of Lung Cancer Cell Lines: Correlation Between Their Production and the Inflammatory/Immunological Responses Both in Vivo and in Vitro  Cancer Sci  2007  98 (7), 1048-54  PubMed ID: 17511773   DOI: 10.1111/j.1349-7006.2007.00507.x
8988  Takashi Fukuyama, Takeshi Hanagiri, Mitsuhiro Takenoyama, Yoshinobu Ichiki, Makiko Mizukami, Tetsuya So, Masakazu Sugaya, Tomoko So, Kenji Sugio, Kosei Yasumoto  Identification of a New Cancer/Germline Gene, KK-LC-1, Encoding an Antigen Recognized by Autologous CTL Induced on Human Lung Adenocarcinoma  Cancer Res  2006  66 (9), 4922-8  PubMed ID: 16651449   DOI: 10.1158/0008-5472.CAN-05-3840
8989  K Sugio, H Uramoto, K Ono, T Oyama, T Hanagiri, M Sugaya, Y Ichiki, T So, S Nakata, M Morita, K Yasumoto  Mutations Within the Tyrosine Kinase Domain of EGFR Gene Specifically Occur in Lung Adenocarcinoma Patients With a Low Exposure of Tobacco Smoking  Br J Cancer  2006  94 (6), 896-903  PubMed ID: 16552419   DOI: 10.1038/sj.bjc.6603040
7413  Mizukami M, Hanagiri T, Baba T, Fukuyama T, Nagata Y, So T, Ichiki Y, Sugaya M, Yasuda M, Takenoyama M, Sugio K, Yasumoto K.  Identification of tumor associated antigens recognized by IgG from tumor-infiltrating B cells of lung cancer: correlation between Ab titer of the patient's sera and the clinical course.  Cancer Sci  2005  96(12):882-8  PubMed ID: 16367908   DOI: 10.1111/j.1349-7006.2005.00119.x
8986  Tetsuya So, Mitsuhiro Takenoyama, Yoshinobu Ichiki, Makiko Mizukami, Tomoko So, Takeshi Hanagiri, Kenji Sugio, Kosei Yasumoto  A Different Pattern of Cytotoxic T Lymphocyte Recognition Against Primary and Metastatic Tumor Cells in a Patient With Nonsmall Cell Lung Carcinoma  Cancer  2005  103 (1), 200-8 2005  PubMed ID: 15573370   DOI: 10.1002/cncr.20782

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