Cell No. : Cell Name
RCB3522 : HAM2
update : 2022/05/30
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| Comment | Human cell line derived from ameloblastoma. RCB3521 was derived from the same patient. Application consideration |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Oral Pathol Med 2011;40:227-234) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Hatakeyama, Setsuko
|
| Originator |
Hatakeyama, Setsuko
|
| Year of deposit |
2010
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Mongoloid
|
| Gender |
Male
|
| Age at sampling |
26 years
|
| Tissue |
maxilla
|
| Disease name |
ameloblastoma
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
pMXpuro-hTERT, PSRαMSV CDK4TKneo
|
| Year of origin |
2007
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
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|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
Defined Keratinocyte-SFM
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin + (0.02% EDTA or 0.04% EDTA), washed twice with PBS(-)
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Culture information
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Passage ratio |
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1 : 2-3 split
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| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
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| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
CELLBANKER 2
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| Freezing method |
|
Slow freezing
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| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| STR(human) |
|
OK
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| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
3205
Taichi Ishikawa, Jun Terashima, Yu Shimoyama, Yu Ohashi, Toshinari Mikami, Yasunori Takeda, Minoru Sasaki
Effects of butyric acid, a bacterial metabolite, on the migration of ameloblastoma mediated by laminin 332
J Oral Sci
2020
62(4):435-438
PubMed ID: 32879156
DOI: 10.2334/josnusd.19-0380
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15610
Ishikawa T, Terashima J, Sasaki D, Shimoyama Y, Yaegashi T, Sasaki M.
Establishment and use of a three-dimensional ameloblastoma culture model to study the effects of butyric acid on the transcription of growth factors and laminin β3
Arch Oral Biol
2020
118:104845
PubMed ID: 32712305
DOI: 10.1016/j.archoralbio.2020.104845
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903
Hatakeyama S, Mizusawa N, Tsutsumi R, Yoshimoto K, Mizuki H, Yasumoto S, Sato S, Takeda Y.
Establishment of human dental epithelial cell lines expressing ameloblastin and enamelin
J Oral Pathol Med
2011
40(3):227-34
PubMed ID: 20923449
DOI: 10.1111/j.1600-0714.2010.00950.x
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