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Cell No. : Cell Name
RCB3521 : HAM1  update : 2022/05/30
CommentHuman cell line derived from ameloblastoma. RCB3522 was derived from the same patient. Application consideration
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Oral Pathol Med 2011;40:227-234) designated by the DEPOSITOR is required.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hatakeyama, Setsuko
Originator Hatakeyama, Setsuko
Year of deposit 2010
Animal human < Mammals
Genus Homo
Species sapiens
Race Mongoloid
Gender Male
Age at sampling 26 years
Tissue maxilla
Disease name ameloblastoma
Classification transformed
Recombinant recombinant
Exogene pMXpuro-hTERT, PSRαMSV CDK4TKneo
Year of origin 2007
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives Defined Keratinocyte-SFM
Antibiotics Free
Passage method 0.25% Trypsin + + (0.02% EDTA or 0.04% EDTA), washed twice with PBS(-)
Culture information Passage ratio 1 : 2-3 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium CELLBANKER 2
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
deposit info
lot info
Reference information Reference 3
User's Publication 0

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3205  Taichi Ishikawa, Jun Terashima, Yu Shimoyama, Yu Ohashi, Toshinari Mikami, Yasunori Takeda, Minoru Sasaki   Effects of butyric acid, a bacterial metabolite, on the migration of ameloblastoma mediated by laminin 332  J Oral Sci  2020  62(4):435-438  PubMed ID: 32879156   DOI: 10.2334/josnusd.19-0380
15610  Ishikawa T, Terashima J, Sasaki D, Shimoyama Y, Yaegashi T, Sasaki M.  Establishment and use of a three-dimensional ameloblastoma culture model to study the effects of butyric acid on the transcription of growth factors and laminin β3  Arch Oral Biol  2020  118:104845  PubMed ID: 32712305   DOI: 10.1016/j.archoralbio.2020.104845
903  Hatakeyama S, Mizusawa N, Tsutsumi R, Yoshimoto K, Mizuki H, Yasumoto S, Sato S, Takeda Y.  Establishment of human dental epithelial cell lines expressing ameloblastin and enamelin   J Oral Pathol Med  2011  40(3):227-34  PubMed ID: 20923449   DOI: 10.1111/j.1600-0714.2010.00950.x

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