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Cell No. : Cell Name
RCB3520 : KM-S  update : 2022/03/28
CommentLung cancer, Immature large cell type
Comment from the depositor
Terms and conditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KAWANAMI, Takafumi
Originator MASAKi, Yasufumi
Year of deposit 2010
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 73 years
Tissue lymph node
Primary focus lung
Metastastatic ability Yes
Metastatsd tissue Lymph node, Prostate
Classification cancer
Year of origin 2004
Lifespan infinite
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Medium and additives RPMI1640+20%FBS RPMI1640 + 20% FBS
Antibiotics Penicillin (100U/ml), Streptomycin (100 μg/ml) Free
Passage method 0.25% Trypsin
Culture information Passage cell No 5x10^(5) cells/ml in 6well plates
Passage ratio 1 : 4-8 split
SC frequency Subculture : 1-2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
STR(human) OK
Reference information Reference 0
User's Publication 0


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