Cell No. : Cell Name
RCB3520 : KM-S
update : 2024/01/17
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| Comment | Lung cancer, Immature large cell type |
| Comment from the depositor | |
| Terms and conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
KAWANAMI, Takafumi
|
| Originator |
MASAKi, Yasufumi
|
| Year of deposit |
2010
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
73 years
|
| Tissue |
lymph node
|
| Primary focus |
lung
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
Lymph node, Prostate
|
| Classification |
cancer
|
| Year of origin |
2004
|
|
Lifespan |
infinite
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
Adherent cells
|
Adherent cells
|
| Culture medium |
RPMI1640+20%FBS
|
RPMI1640 + 20% FBS
|
| Antibiotics |
Penicillin (100U/ml), Streptomycin (100 μg/ml)
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
5x10 5 cells/ml in 6well plates
|
|
| Passage ratio |
|
1 : 4-8 split
|
| SC frequency |
Subculture : 1-2 times/week
|
Subculture : 2 times/week
|
| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
0
|
| User's Publication |
0
|
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