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Cell No. : Cell Name
RCB3518 : KM-Y  update : 2022/03/28
CommentBurkitt lymphoma.
Comment from the depositor
Terms and conditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KAWANAMI, Tatafumi
Originator MASAKI, Yasufumi
Year of deposit 2010
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 75 years
Tissue peripheral blood lymphocytes
Disease name Burkitt lymphoma
Metastastatic ability Yes
Metastatsd tissue Pleural effusion, Bone marrow, Stomach, Spleen
Genetics t(8;14)(q24;q32), t(14:18)(q32;q21)
Classification cancer
Year of origin 2003
Lifespan infinite
Morphology lymphocyte-like
Surface antigen (depositor) CD19, CD20, HLA-DR, IgGλ
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives RPMI1640 + 20%FBS RPMI1640 + 20% FBS
Antibiotics Penicillin (100U/ml), Streptomycin (100 μg/ml) Free
Passage method dilution
Culture information Passage cell No 5x10^(5) cells/ml in 6well plates
Passage ratio 1 : 10-20 split
SC frequency Subculture : 1-2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium CELLBANKER Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) Undetected
Virus (HTLV-1) (-)
Virus (EBV) (+) (+)
STR(human) OK
Reference information Reference 0
User's Publication 0


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