Cell No. : Cell Name
RCB3518 : KM-Y
update : 2024/11/11
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| Comment | Burkitt lymphoma. |
| Comment from the depositor | |
| Terms and conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
KAWANAMI, Tatafumi
|
| Originator |
MASAKI, Yasufumi
|
| Year of deposit |
2010
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
75 years
|
| Tissue |
peripheral blood lymphocytes
|
| Disease name |
Burkitt lymphoma
|
| Metastatic ability |
Yes
|
| Metastatic tissue |
Pleural effusion, Bone marrow, Stomach, Spleen
|
| Genetics |
t(8;14)(q24;q32), t(14:18)(q32;q21)
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| Classification |
cancer
|
| Year of origin |
2003
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
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| Surface antigen (depositor) |
CD19, CD20, HLA-DR, IgGλ
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|
| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
Suspension cells
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
RPMI1640 + 20%FBS
|
RPMI1640 + 20% FBS
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| Antibiotics |
Penicillin (100U/ml), Streptomycin (100 μg/ml)
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Free
|
|
Passage method |
|
dilution
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Culture information
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Passage cell No |
5x10 5 cells/ml in 6well plates
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|
| Passage ratio |
|
1 : 10-20 split
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| SC frequency |
Subculture : 1-2 times/week
|
Subculture : 2 times/week
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| Temperature |
37
℃
|
37
℃
|
| CO2 concentration |
5
%
|
5
%
|
| Freeze medium |
CELLBANKER
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
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Undetected
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| Virus (HTLV-1) |
|
(-)
|
| Virus (EBV) |
(+)
|
(+)
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| STR(human) |
|
OK
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| Reference information |
Reference |
0
|
| User's Publication |
0
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