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Cell No. : Cell Name
RCB3517 : LLKF  update : 2022/05/30
CommentA cell line derived from kidney of steppe lemming.
Comment from the depositor
Terms and conditions1) The RECIPIENT will be limited to Academic or public research institute only. 2) The MATERIAL will be used for teaching or not-for-profit research purpose inly. Research shall not include any research conducted by the RECipient (a) for the benefiting of or on behalf of any third or (b) subject to a licensing requirement, a consulting obligation or other similar obligation to a third party with respect to such research. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor KUBO, Yoshinao
Originator KUBO, Yoshinao
Year of deposit 2010
Animal steppe lemming < Mammals
Genus Lagurus
Species lagurus
Gender Male
Tissue kidney
Classification normal
Lifespan infinite
Cellosaurus(Expasy) CVCL_A6SF
deposit info
lot info
Medium Medium List
Culture type Adherent cells Adherent cells
Medium and additives D-MEM + 10% FBS DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 2 split 1 : 2 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Reference information Reference 0
User's Publication 0


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