Cell No. : Cell Name
RCB3515 : JLS-V9 BudR
update : 2023/10/16
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| Comment | Mouse cell line derived from bone marrow. Fibroblast-like. BudR resistant. BALB/c strain. TKG0356 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Fukuda, Hiroshi
|
| Originator |
Wright, B. S.
|
| Year of deposit |
2010
|
| Original cell |
TKG0356
|
| Animal |
_mouse
< Mammals
|
| Strain name |
BALB/c
|
| Age at sampling |
weanling
|
| Tissue |
bone marrow, tibia femur
|
| Classification |
other
|
| History |
Tohoku University（TKG0356）
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_F944
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
RPMI1640 + 10% FBS
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
|
| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
6
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| User's Publication |
0
|
| Reference |
|
5245
Perry A, Robinson OR Jr, Shibley GP.
Enhanced production of Rauscher leukemia virus after infection of high-passaged JLS-V9 cells.
Appl Environ Microbiol
1978
36(3):526-9
PubMed ID: 569463
DOI: 10.1128/aem.36.3.526-529.1978
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5244
Johnson RW, Perry A, Robinson OR Jr, Shibley GP.
Method for reproducible large-volume production and purification of Rauscher murine leukemia virus.
Appl Environ Microbiol
1976
31(2):182-8
PubMed ID: 63267
DOI: 10.1128/aem.31.2.182-188.1976
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5243
Hodge HM, Klein F, Bandyopadhyay AK, Robinson OR Jr, Shibley GP.
Adaptation and infection of mouse bone marrow (JLS-V9) cells in suspension culture for production of Rauscher leukemia virus.
Appl Microbiol
1974
27(1):224-31
PubMed ID: 4129475
DOI: 10.1128/am.27.1.224-231.1974
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5242
Wright BS, O'Brien PA, Stephens R, Bielewicz H, Shibley GP.
Growth curves of Rauscher and Friend murine leukemia viruses in JLS-V9 tissue culture.
Cancer Res
1968
28(8):1655-8
PubMed ID: 5673573
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5240
Chopra HC, Shibley GP.
Infection of an established mouse cell line with cell-free Rauscher leukemia virus. I. Cytological study with the electron microscope.
J Natl Cancer Inst
1967
39(2):241-55
PubMed ID: 18623942
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5241
Wright BS, O'Brien PA, Shibley GP, Mayyasi SA, Lasfargues JC.
Infection of an established mouse bone marrow cell line (JLS-V9) with Rauscher and Moloney murine leukemia viruses.
Cancer Res
1967
27(9):1672-7
PubMed ID: 6057678
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