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Cell No. : Cell Name
RCB3052 : Jurkat  update : 2024/07/11
CommentHuman T cell leukemia. IL-2 receptor (-). Progeny were cured by Treatments with BM Cycline and MC210. TKG0209 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2010
Original cell TKG0209
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 14 years
Tissue blood
Disease name acute T cell leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0209)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0065
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8-15 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Virus (HIV) Lot.1 : (-)
Virus (HTLV-1) Lot.1 : (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Relational File deposit infolot info
Reference information Reference 7
User's Publication 26

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1640  LaGree KA, Lee AT, Stetten G, Strauss PR.  The human Jurkat (FHCRC-11) cell line is heterogeneous in ploidy and cell size and releases detergent-soluble DNA.  Exp Hematol  1988  16(8):686-90  PubMed ID: 3165345  
4749  Wendler I1, Jentsch KD, Schneider J, Hunsmann G.  Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells.  Med Microbiol Immunol  1987  176(5):273-80  PubMed ID: 2443825   DOI: 10.1007/BF00190533
1685  Weiss A, Wiskocil RL, Stobo JD.  The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL 2 production reflects events occurring at a pre-translational level.  J Immunol  1984  133(1):123-8  PubMed ID: 6327821  
1638  Pawelec G, Borowitz A, Krammer PH, Wernet P.  Constitutive interleukin 2 production by the JURKAT human leukemic T cell line.  Eur J Immunol  1982  12(5):387-92  PubMed ID: 6980126   DOI: 10.1002/eji.1830120506
1637  Kahle P, Wernet P, Rehbein A, Kumbier I, Pawelec G.  Cloning of functional human T lymphocytes by limiting dilution: impact of filler cells and interleukin 2 sources on cloning efficiencies.  Scand J Immunol  1981  14(5):493-502  PubMed ID: 6278580  
515  Gillis S, Watson J.  Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.  J Exp Med  1980  152(6):1709-19  PubMed ID: 6778951   DOI: 10.1084/jem.152.6.1709
902  Schneider U, Schwenk HU, Bornkamm G.  Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma.  Int J Cancer  1977  19(5):621-6  PubMed ID: 68013   DOI: 10.1002/ijc.2910190505

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User's Publication
21826  Terasaki T, Semba Y, Sasaki K, Imanaga H, Setoguchi K, Yamauchi T, Hirabayashi S, Nakao F, Akahane K, Inukai T, Sanda T, Akashi K, Maeda T.  The RNA helicases DDX19A/B modulate selinexor sensitivity by regulating MCL1 mRNA nuclear export in leukemia cells.  Leukemia  2024    PubMed ID: 38987275   DOI: 10.1038/s41375-024-02343-2
19947  Moriizumi H, Kubota Y, Tsuchiya T, Naka R, Takekawa M.  Caspase 3-specific cleavage of MEK1 suppresses ERK signaling and sensitizes cells to stress-induced apoptosis  FEBS Open Bio  2023  13(4):684-700  PubMed ID: 36776127   DOI: 10.1002/2211-5463.13574
21095  Pavillon N, Smith NI.  Non-invasive monitoring of T cell differentiation through Raman spectroscopy.  Sci Rep  2023  13(1):3129  PubMed ID: 36813799   DOI: 10.1038/s41598-023-29259-8
21386  Kato N, Kozako T, Ohsugi T, Uchida Y, Yoshimitsu M, Ishitsuka K, Aikawa A, Honda SI.  CDK9 Inhibitor Induces Apoptosis, Autophagy, and Suppression of Tumor Growth in Adult T-Cell Leukemia/Lymphoma.  Biol Pharm Bull  2023  46(9):1269-1276  PubMed ID: 37661406   DOI: 10.1248/bpb.b23-00228
21503  Ijima S, Saito Y, Yamamoto S, Nagaoka K, Iwamoto T, Kita A, Miyajima M, Sato T, Miyazaki A, Chikenji TS.  Senescence-associated secretory phenotypes in mesenchymal cells contribute to cytotoxic immune response in oral lichen planus.  Immun Ageing  2023  20(1):72  PubMed ID: 38053160   DOI: 10.1186/s12979-023-00400-5
21524  Aikawa A, Kozako T, Kato N, Ohsugi T, Honda SI.  Anti-tumor activity of 5-aminoimidazole-4-carboxamide riboside with AMPK-independent cell death in human adult T-cell leukemia/lymphoma.  Eur J Pharmacol  2023  961:176180  PubMed ID: 37956732   DOI: 10.1016/j.ejphar.2023.176180
17711  Yamaguchi S, Ikeda R, Umeda Y, Kosaka T, Yamahira S, Okamoto A.  Chemoenzymatic Labeling to Visualize Intercellular Contacts Using Lipidated SortaseA  Chembiochem  2022  23(21):e202200474  PubMed ID: 35976800   DOI: 10.1002/cbic.202200474
20533  Kozako T, Kato N, Ohsugi T, Uchida YI, Yoshimitsu M, Ishitsuka K, Higaki Y, Sato H, Aikawa A, Honda SI.  SRT1720 induces SIRT1-independent cell death in adult T-cell leukemia/lymphoma.  FEBS J  2022    PubMed ID: 35029032   DOI: 10.1111/febs.16353
20746  Koba K, Yarimizu K, Fujiyoshi S, Oshiro K, Wakizaka Y, Takano M, Maruyama F.  Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip.  STAR Protoc  2022  3(3):101527  PubMed ID: 35779257   DOI: 10.1016/j.xpro.2022.101527
20768  Sakakibara M, Sumida H, Yanagida K, Miyasato S, Nakamura M, Sato S.  Bitter taste receptor T2R38 is expressed on skin-infiltrating lymphocytes and regulates lymphocyte migration.  Sci Rep  2022  12(1):11790  PubMed ID: 35821061   DOI: 10.1038/s41598-022-15999-6
20935  Deng H, Lei Q, Wang C, Wang Z, Chen H, Wang G, Yang N, Huang D, Yu Q, Yao M, Xiao X, Zhu G, Cheng C, Li Y, Li F, Tian P, Li W.  A fluorogenic probe for predicting treatment response in non-small cell lung cancer with EGFR-activating mutations.  Nat Commun  2022  13(1):6944  PubMed ID: 36376325   DOI: 10.1038/s41467-022-34627-5
13356  Takheaw N, Sittithumcharee G, Kariya R, Kasinrerk W, Okada S.  Anti-human CD99 antibody exerts potent antitumor effects in mantle cell lymphoma.  Cancer Immunol Immunother  2021  70(6):1557-1567  PubMed ID: 33215253   DOI: 10.1007/s00262-020-02789-0
14478  Yamanaka D, Shoda M, Matsubara T, Ishibashi KI, Motoi A, Motoi R, Motoi M, Ohno N, Adachi Y.  Open-Label Study of the Influence of Food Containing the Royal Sun Mushroom, Agaricus brasiliensis KA21 (Agaricomycetes), on β-Glucan-Specific Antibody Production in Healthy Human Volunteers  Int J Med Mushrooms  2021  23(2):13-28  PubMed ID: 33639078   DOI: 10.1615/IntJMedMushrooms.2020037471
20229  Uesugi S, Hakozaki M, Kanno Y, Takahashi H, Kudo Y, Kimura KI, Yamada H, Yano A.  A yeast-based screening system identified bakkenolide B contained in Petasites japonicus as an inhibitor of interleukin-2 production in a human T cell line.  Biosci Biotechnol Biochem  2021    PubMed ID: 34251393   DOI: 10.1093/bbb/zbab130
20439  Yamagishi M, Kubokawa M, Kuze Y, Suzuki A, Yokomizo A, Kobayashi S, Nakashima M, Makiyama J, Iwanaga M, Fukuda T, Watanabe T, Suzuki Y, Uchimaru K.  Chronological genome and single-cell transcriptome integration characterizes the evolutionary process of adult T cell leukemia-lymphoma.  Nat Commun  2021  12(1):4821  PubMed ID: 34376672   DOI: 10.1038/s41467-021-25101-9
16841  Shimoyama S, Kawata K, Ohta K, Chida T, Suzuki T, Tsuneyama K, Shimoda S, Kurono N, Leung PSC, Gershwin ME, Suda T, Kobayashi Y.  Ursodeoxycholic acid impairs liver-infiltrating T-cell chemotaxis through IFN-γ and CX3CL1 production in primary biliary cholangitis.  Eur J Immunol  2021  51(6):1519-1530  PubMed ID: 33710617   DOI: 10.1002/eji.202048589
6947  Araki R1, Hoki Y, Suga T, Obara C, Sunayama M, Imadome K, Fujita M, Kamimura S, Nakamura M, Wakayama S, Nagy A, Wakayama T, Abe M.  Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency.  Nat Commun  2020  11(1):197  PubMed ID: 31924765   DOI: 10.1038/s41467-019-13830-x
13369  Okimune KI, Nagy SK, Hataya S, Endo Y, Takasuka TE.  Reconstitution of Drosophila and human chromatins by wheat germ cell-free co-expression system.  BMC Biotechnol  2020    PubMed ID: 33261588   DOI: 10.1186/s12896-020-00655-6
11347  Nomura H, Umekita K, Hashikura Y, Umeki K, Yamamoto I, Aratake Y, Saito M, Hasegawa H, Yanagihara K, Okayama A.  Diversity of cell phenotypes among MT-2 cell lines affects the growth of U937 cells and cytokine production.  Hum. Cell  2019    PubMed ID: 30560508   DOI: 10.1007/s13577-018-00231-3
11625  Kurosawa N, Wakata Y, Ida K, Midorikawa A, Isobe M.  High throughput development of TCR-mimic antibody that targets survivin-2B80-88/HLA-A*A24 and its application in a bispecific T-cell engager.  Sci Rep  2019    PubMed ID: 31285464   DOI: 10.1038/s41598-019-46198-5
10440  Shunko A Inada , Daniel ZKW  Evaluation of 389nm UV-LED to Induce Apoptosis in Leukemia Cells for Develop Implant Photonics-Device  Biomedical Journal of Scientific & Technical Research  2019  19:14688-14692B    DOI: 10.26717/BJSTR.2019.19.003379
6902  Kozako T, Mellini P, Ohsugi T, Aikawa A, Uchida YI, Honda SI, Suzuki T.  Novel small molecule SIRT2 inhibitors induce cell death in leukemic cell lines.  BMC Cancer  2018    PubMed ID: 30081901   DOI: 10.1186/s12885-018-4710-1
10061  Hashikura Y, Umeki K, Umekita K, Nomura H, Yamada A, Yamamoto I, Hasegawa H, Yanagihara K, Okayama A.  Infection of defective human T-lymphotropic virus type 1.  Hum. Cell  2017  30:117-123  PubMed ID: 28070874   DOI: 10.1007/s13577-016-0156-4
9532  Horie Y, Nemoto H, Itoh M, Kosaka H, Morita K.  Fermented Brown Rice Extract Causes Apoptotic Death of Human Acute Lymphoblastic Leukemia Cells via Death Receptor Pathway.  Appl. Biochem. Biotechnol.  2016  178:1599-611  PubMed ID: 26769704   DOI: 10.1007/s12010-015-1970-y
12490  Naotomo Tottori, Takasi Nisisako, Jongho Park, Yasuko Yanagida, Takeshi Hatsuzawa  Separation of viable and nonviable mammalian cells using a deterministic lateral displacement microfluidic device  Biomicrofluidics  2016  10    DOI: 10.1063/1.4942948
13468  Kawaguchi M, Aoki S, Hirao T, Morita M, Ito K.  Autophagy is an important metabolic pathway to determine leukemia cell survival following suppression of the glycolytic pathway  Biochem Biophys Res Commun  2016  474(1):188-192  PubMed ID: 27107693   DOI: 10.1016/j.bbrc.2016.04.098

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