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Cell No. : Cell Name
RCB3052 : Jurkat  update : 2022/08/15
CommentHuman T cell leukemia. IL-2 receptor (-). Progeny were cured by Treatments with BM Cycline and MC210. TKG0209 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2010
Original cell TKG0209
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 14 years
Tissue blood
Disease name acute T cell leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0209)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0065
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Relational File deposit infolot info
Reference information Reference 7
User's Publication 18

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1640  LaGree KA, Lee AT, Stetten G, Strauss PR.  The human Jurkat (FHCRC-11) cell line is heterogeneous in ploidy and cell size and releases detergent-soluble DNA.  Exp Hematol  1988  16(8):686-90  PubMed ID: 3165345
4749  Wendler I1, Jentsch KD, Schneider J, Hunsmann G.  Efficient replication of HTLV-III and STLV-IIImac in human Jurkat cells.  Med Microbiol Immunol  1987  176(5):273-80  PubMed ID: 2443825
1685  Weiss A, Wiskocil RL, Stobo JD.  The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL 2 production reflects events occurring at a pre-translational level.  J Immunol  1984  133(1):123-8  PubMed ID: 6327821
1638  Pawelec G, Borowitz A, Krammer PH, Wernet P.  Constitutive interleukin 2 production by the JURKAT human leukemic T cell line.  Eur J Immunol  1982  12(5):387-92  PubMed ID: 6980126
1637  Kahle P, Wernet P, Rehbein A, Kumbier I, Pawelec G.  Cloning of functional human T lymphocytes by limiting dilution: impact of filler cells and interleukin 2 sources on cloning efficiencies.  Scand J Immunol  1981  14(5):493-502  PubMed ID: 6278580
515  Gillis S, Watson J.  Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line.  J Exp Med  1980  152(6):1709-19  PubMed ID: 6778951
902  Schneider U, Schwenk HU, Bornkamm G.  Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma.  Int J Cancer  1977  19(5):621-6  PubMed ID: 68013

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User's Publication
20533  Kozako T, Kato N, Ohsugi T, Uchida YI, Yoshimitsu M, Ishitsuka K, Higaki Y, Sato H, Aikawa A, Honda SI.  SRT1720 induces SIRT1-independent cell death in adult T-cell leukemia/lymphoma.  FEBS J  2022    PubMed ID: 35029032
20746  Koba K, Yarimizu K, Fujiyoshi S, Oshiro K, Wakizaka Y, Takano M, Maruyama F.  Protocol of living cell separation using the microfluidic dielectrophoresis integrated chip.  STAR Protoc  2022  3(3):101527  PubMed ID: 35779257
20768  Sakakibara M, Sumida H, Yanagida K, Miyasato S, Nakamura M, Sato S.  Bitter taste receptor T2R38 is expressed on skin-infiltrating lymphocytes and regulates lymphocyte migration.  Sci Rep  2022  12(1):11790  PubMed ID: 35821061
13356  Takheaw N, Sittithumcharee G, Kariya R, Kasinrerk W, Okada S.  Anti-human CD99 antibody exerts potent antitumor effects in mantle cell lymphoma.  Cancer Immunol Immunother  2021  70(6):1557-1567  PubMed ID: 33215253
14478  Yamanaka D, Shoda M, Matsubara T, Ishibashi KI, Motoi A, Motoi R, Motoi M, Ohno N, Adachi Y.  Open-Label Study of the Influence of Food Containing the Royal Sun Mushroom, Agaricus brasiliensis KA21 (Agaricomycetes), on β-Glucan-Specific Antibody Production in Healthy Human Volunteers  Int J Med Mushrooms  2021  23(2):13-28  PubMed ID: 33639078
20229  Uesugi S, Hakozaki M, Kanno Y, Takahashi H, Kudo Y, Kimura KI, Yamada H, Yano A.  A yeast-based screening system identified bakkenolide B contained in Petasites japonicus as an inhibitor of interleukin-2 production in a human T cell line.  Biosci Biotechnol Biochem  2021    PubMed ID: 34251393
20439  Yamagishi M, Kubokawa M, Kuze Y, Suzuki A, Yokomizo A, Kobayashi S, Nakashima M, Makiyama J, Iwanaga M, Fukuda T, Watanabe T, Suzuki Y, Uchimaru K.  Chronological genome and single-cell transcriptome integration characterizes the evolutionary process of adult T cell leukemia-lymphoma.  Nat Commun  2021  12(1):4821  PubMed ID: 34376672
16841  Shimoyama S, Kawata K, Ohta K, Chida T, Suzuki T, Tsuneyama K, Shimoda S, Kurono N, Leung PSC, Gershwin ME, Suda T, Kobayashi Y.  Ursodeoxycholic acid impairs liver-infiltrating T-cell chemotaxis through IFN-γ and CX3CL1 production in primary biliary cholangitis.  Eur J Immunol  2021  51(6):1519-1530  PubMed ID: 33710617
6947  Araki R1, Hoki Y, Suga T, Obara C, Sunayama M, Imadome K, Fujita M, Kamimura S, Nakamura M, Wakayama S, Nagy A, Wakayama T, Abe M.  Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency.  Nat Commun  2020  11(1):197  PubMed ID: 31924765
13369  Okimune KI, Nagy SK, Hataya S, Endo Y, Takasuka TE.  Reconstitution of Drosophila and human chromatins by wheat germ cell-free co-expression system.  BMC Biotechnol  2020    PubMed ID: 33261588
11347  Nomura H, Umekita K, Hashikura Y, Umeki K, Yamamoto I, Aratake Y, Saito M, Hasegawa H, Yanagihara K, Okayama A.  Diversity of cell phenotypes among MT-2 cell lines affects the growth of U937 cells and cytokine production.  Hum. Cell  2019    PubMed ID: 30560508
11625  Kurosawa N, Wakata Y, Ida K, Midorikawa A, Isobe M.  High throughput development of TCR-mimic antibody that targets survivin-2B80-88/HLA-A*A24 and its application in a bispecific T-cell engager.  Sci Rep  2019    PubMed ID: 31285464
10440  Shunko A Inada , Daniel ZKW  Evaluation of 389nm UV-LED to Induce Apoptosis in Leukemia Cells for Develop Implant Photonics-Device  Biomedical Journal of Scientific & Technical Research  2019  19:14688-14692B 
6902  Kozako T, Mellini P, Ohsugi T, Aikawa A, Uchida YI, Honda SI, Suzuki T.  Novel small molecule SIRT2 inhibitors induce cell death in leukemic cell lines.  BMC Cancer  2018    PubMed ID: 30081901
10061  Hashikura Y, Umeki K, Umekita K, Nomura H, Yamada A, Yamamoto I, Hasegawa H, Yanagihara K, Okayama A.  Infection of defective human T-lymphotropic virus type 1.  Hum. Cell  2017  30:117-123  PubMed ID: 28070874
9532  Horie Y, Nemoto H, Itoh M, Kosaka H, Morita K.  Fermented Brown Rice Extract Causes Apoptotic Death of Human Acute Lymphoblastic Leukemia Cells via Death Receptor Pathway.  Appl. Biochem. Biotechnol.  2016  178:1599-611  PubMed ID: 26769704
12490  Naotomo Tottori, Takasi Nisisako, Jongho Park, Yasuko Yanagida, Takeshi Hatsuzawa  Separation of viable and nonviable mammalian cells using a deterministic lateral displacement microfluidic device  Biomicrofluidics  2016  10 
13468  Kawaguchi M, Aoki S, Hirao T, Morita M, Ito K.  Autophagy is an important metabolic pathway to determine leukemia cell survival following suppression of the glycolytic pathway  Biochem Biophys Res Commun  2016  474(1):188-192  PubMed ID: 27107693

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